Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)
Rabbit ACE2 Polyclonal Antibody | anti-ACE2 antibody
ACE2 Antibody
IHC: 2 ug/mL
IF: 10 ug/mL
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human, mouse andrat samples. All other applications and species not yet tested.
Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)
Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of ACE2 inHuman Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3227) at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.)
Immunofluorescence (IFC)
(Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with MBS151315 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
Immunofluorescence (IFC)
(Figure 10 Immunofluorescence Validation of ACE2 in Human Kidney Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with MBS151315 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
Testing Data (TD)
(Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS150723 (2 ug/mL), ACE2, MBS150187 (2 ug/mL), ACE2, MBS151315 (2 ug/mL) and beta-actin MBS150383(1 ug)
Testing Data (TD)
(Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS150723 (2 ug/mL), ACE2, MBS150187 (2 ug/mL), ACE2, MBS151315 (2 ug/mL) and beta-actin MBS150383(1 ug)
Western Blot (WB)
(Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 2 Western Blot Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 4 Western Blot Validation in Rat Thymus Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 4 Western Blot Validation in Rat Thymus Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2, MBS151315(2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Immunofluorescent (IFC)
(Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescent (IFC)
(Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 8 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 8 Immunofluorescence Validation of ACE2 in Mouse Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with MBS151315 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 9 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with MBS151315 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 9 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with MBS151315 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IFC)
(Figure 11 Immunofluorescence Validation of ACE2 In Caco2 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with MBS151315 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
Immunofluorescence (IFC)
(Figure 11 Immunofluorescence Validation of ACE2 In Caco2 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with MBS151315 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
NCBI and Uniprot Product Information
Observed MW: 130 kD (7 N-linked glycostation)
NCBI Description
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Uniprot Description
ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 3.4.17.23; Protease
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; plasma membrane; integral to membrane; lipid raft
Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; zinc ion binding; carboxypeptidase activity; metallopeptidase activity; peptide hormone binding; endopeptidase activity; glycoprotein binding
Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; proteolysis; angiotensin maturation; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cell proliferation; regulation of cytokine production; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior
Research Articles on ACE2
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Product Notes
The ACE2 ace2 (Catalog #AAA151315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACE2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ACE2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF). WB: 1-2 ug/mL IHC: 2 ug/mL IF: 10 ug/mL Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human, mouse andrat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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