Western Blot (WB)
( Anti-ROR1 Antibody at 1:4000 dilution +PANC-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse ROR1 Polyclonal Antibody | anti-ROR1 antibody
ROR1 Antibody
WB~~1:4000
FC~~1:25
Western Blot (WB)
( Anti-ROR1 Antibody at 1:4000 dilution +PANC-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
( Anti-ROR1 Antibody at 1:4000 dilution +PANC-1 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
( All lanes : Anti-ROR1 Antibody at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2:MDA-MB-231 whole cell lysate Lane 3:NCI-H226 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
( All lanes : Anti-ROR1 Antibody at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2:MDA-MB-231 whole cell lysate Lane 3:NCI-H226 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC)
( Overlay histogram showing A549 cells stained with MBS9200685 (green line). The cells were fixedwith 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serumalbumin to block non-specific protein-protein interactions followed by the antibody (MBS9200685, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC)
( Overlay histogram showing A549 cells stained with MBS9200685 (green line). The cells were fixedwith 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serumalbumin to block non-specific protein-protein interactions followed by the antibody (MBS9200685, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Immunohistochemistry (IHC)
( MBS9200685 staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
( MBS9200685 staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
( MBS9200685 staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
( MBS9200685 staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
( Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
( Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]
Uniprot Description
ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Ror family
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex
Molecular Function: Wnt-protein binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on ROR1
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Product Notes
The ROR1 ror1 (Catalog #AAA9200685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROR1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ROR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P). IHC-P~~1:10~50 WB~~1:4000 FC~~1:25. Researchers should empirically determine the suitability of the ROR1 ror1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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