Rabbit anti-Human, Mouse MYD88 Polyclonal Antibody | anti-MYD88 antibody

MYD88 Antibody

Cat. Num. AAA150754

• Gene Names: MYD88; MYD88D
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: MYD88 Antibody is affinity chromatography purified via peptide column.
• Synonyms: MYD88; Polyclonal Antibody; MYD88 Antibody; Myeloid differentiation primary response 88; MYD88D; myeloid differentiation primary response gene (88); anti-MYD88 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: MYD88 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 2682

• Applicable Applications for anti-MYD88 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: MYD88 antibody can be used for detection of MyD88 by Western blot 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: MD88 antibody was raised against a 16 amino acid peptide near the center of human MD88.
• Buffer: MYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: MYD88 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Figure 1 Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) CellsLoading: 15 μg of lysates per lane.Antibodies: 2125 (1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Western Blot (WB)

(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: MyD88 2125 (2 μg/mL), MyD88 2127 (2 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Western Blot (WB)

(Figure 3 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 μg of lysates per lane.Antibodies: MyD88 2125 (2 μg/mL), MyD88 2127 (2 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Western Blot (WB)

(Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 15 μg per lane.Antibodies: 2125 (2 μg/mL) or 2127 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Western Blot (WB)

(Figure 5 Validation with MyD88 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or MyD88 siRNAs (lane 2)Loading: 10 μg of HeLa whole cell lysates per lane.Antibodies: 2125 (2 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Immunofluorescence (IF)

(Figure 6 Immunofluorescence Validation of MyD88Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MyD88 with 2125 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing nucleus staining on human testis cells.)

Immunohistochemistry (IHC)

(Figure 7 Immunohistochemistry Validation of MyD88Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-MyD88 antibody (2125) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

Immunofluorescence (IF)

(Figure 8 Immunofluorescence Validation of MyD88 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling MyD88 with 2125 at 10 μg/mL, followed by Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

Western Blot (WB)

(Figure 9 KO Validation in Macrophages (Miller et al., 2006)Bone marrow-derived macrophages from wild type (WT) mice and MyD88 knockout mice were assessed for MyD88 protein expression by anti-MyD88 antibodies. MyD88 expression was detected in WT mice, but not in MyD88 knockout mice.)

Western Blot (WB)

(Figure 10 KO Validation in MyD88-deficient MEF cell line (Burns et al., 2003)MyD88?/?-deficient MEF cell line was reconstituted by retroviral infection with an empty vector, MyD88, or MyD88s expression vectors. The levels of MyD88 (isoform 1) or MyD88s (isoform 3) were confirmed with anti-MyD88 antibodies and MyD88 expression was not detected in the MyD88-deficient cells.)

Western Blot (WB)

(Figure 11 KO Validation in IECs (Vlantis et al., 2014)Western blot with anti-MyD88 antibodies on intestinal epithelial cells (IECs) showing efficient deletion of MyD88 and concomitant expression of GFP in MyD88IEC-KO mice, but not in MyD88 knockout mice.)

Western Blot (WB)

(Figure 12 KD Validation in Chondrocytes (Ahmad et al., 2009)Chondrocytes were transfected with either MyD88 siRNA or control siRNA and analyzed for MyD88 expression by immunoblotting with anti-Myd88 antibodies that confirmed inhibition of the target proteins.)

Immunoprecipitation (IP)

(Figure 13 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)

Related Product Information for anti-MYD88 antibody

MYD88 Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 1763090
• NCBI GeneID: 4615
• UniProt Accession #: U70451; Q99836
• Molecular Weight: 35 kDa
• NCBI Official Full Name: Human myleoid differentiation primary response protein MyD88 mRNA, complete cds
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88; myeloid differentiation primary response gene (88)
• Protein Family: Myeloid differentiation primary response protein

NCBI Description

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: plasma membrane; endosome membrane; cytosol

Molecular Function: identical protein binding; protein binding; death receptor binding

Biological Process: positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; regulation of inflammatory response; positive regulation of interferon type I production; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency