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Western Blot (WB)
(Western blot analysis of lysate from A2058 cell line, using p16INK4a Antibody (C-term E119). MBS9207467 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit anti-Human p16INK4a Polyclonal Antibody | anti-CDKN2A antibody
p16INK4a Antibody (C-term E119)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
p16INK4a; Polyclonal Antibody; p16INK4a Antibody (C-term E119); Cyclin-dependent kinase inhibitor 2A; isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A; CDKN2A; CDKN2; MTS1; anti-CDKN2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This p16INK4a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the C-terminal region of human p16INK4a.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.27 (varies by lot)
Sequence Positions
104-131
Sequence Length
85
Applicable Applications for anti-CDKN2A antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from A2058 cell line, using p16INK4a Antibody (C-term E119). MBS9207467 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from A2058 cell line, using p16INK4a Antibody (C-term E119). MBS9207467 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Immunohistochemistry (IHC)
(p16INK4a Antibody (C-term E119) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p16INK4a Antibody (C-term E119) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(p16INK4a Antibody (C-term E119) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p16INK4a Antibody (C-term E119) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(p16INK4a Antibody (C-term E119) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(p16INK4a Antibody (C-term E119) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of p16INK4a Antibody (C-term E119) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of p16INK4a Antibody (C-term E119) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-CDKN2A antibody
This gene generates several transcript variants which
differ in their first exons. At least three alternatively spliced
variants encoding distinct proteins have been reported, two of
which encode structurally related isoforms known to function as
inhibitors of CDK4 kinase. The remaining transcript includes an
alternate first exon located 20 Kb upstream of the remainder of the
gene; this transcript contains an alternate open reading frame
(ARF) that specifies a protein which is structurally unrelated to
the products of the other variants. This ARF product functions as a
stabilizer of the tumor suppressor protein p53 as it can interact
with, and sequester, MDM1, a protein responsible for the
degradation of p53. In spite of the structural and functional
differences, the CDK inhibitor isoforms and the ARF product encoded
by this gene, through the regulatory roles of CDK4 and p53 in cell
cycle G1 progression, share a common functionality in cell cycle G1
control. This gene is frequently mutated or deleted in a wide
variety of tumors, and is known to be an important tumor suppressor
gene.
differ in their first exons. At least three alternatively spliced
variants encoding distinct proteins have been reported, two of
which encode structurally related isoforms known to function as
inhibitors of CDK4 kinase. The remaining transcript includes an
alternate first exon located 20 Kb upstream of the remainder of the
gene; this transcript contains an alternate open reading frame
(ARF) that specifies a protein which is structurally unrelated to
the products of the other variants. This ARF product functions as a
stabilizer of the tumor suppressor protein p53 as it can interact
with, and sequester, MDM1, a protein responsible for the
degradation of p53. In spite of the structural and functional
differences, the CDK inhibitor isoforms and the ARF product encoded
by this gene, through the regulatory roles of CDK4 and p53 in cell
cycle G1 progression, share a common functionality in cell cycle G1
control. This gene is frequently mutated or deleted in a wide
variety of tumors, and is known to be an important tumor suppressor
gene.
Product Categories/Family for anti-CDKN2A antibody
References
Kovacs, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(12):5429-5434(2010)
Irvine, M., et al. Cell Cycle 9(4):829-839(2010)
Zhang, H.J., et al. J. Cell. Biochem. 106(3):464-472(2009)
Ivanchuk, S.M., et al. Cell Cycle 7(12):1836-1850(2008)
Bandyopadhyay, K., et al. Biochemistry 46(49):14325-14334(2007)
Irvine, M., et al. Cell Cycle 9(4):829-839(2010)
Zhang, H.J., et al. J. Cell. Biochem. 106(3):464-472(2009)
Ivanchuk, S.M., et al. Cell Cycle 7(12):1836-1850(2008)
Bandyopadhyay, K., et al. Biochemistry 46(49):14325-14334(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16533
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform p16INK4a
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
CDKN2A
NCBI Official Synonym Symbols
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A
UniProt Protein Name
Cyclin-dependent kinase inhibitor 2A
Protein Family
UniProt Gene Name
CDKN2A
UniProt Synonym Gene Names
CDK4I; MTS-1; p16-INK4; p16INK4A
UniProt Entry Name
CDN2A_HUMAN
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Product Notes
The CDKN2A cdkn2a (Catalog #AAA9207467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-131. The p16INK4a Antibody (C-term E119) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p16INK4a can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the CDKN2A cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16INK4a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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