Test Data
IgG (H&L) Secondary Antibody
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG
Reactivity
Rabbit.Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Affinity purified antibody is > 95% based on SDS-PAGEAffinity purified using solid phase Rabbit IgG.
Synonyms
IgG (H&L); Donkey anti-Rabbit IgG (H&L) - Affinity Pure; DyLight350 Conjugate; min x w/bovine; chicken; goat; guinea pig; hamster; horse; human; mouse; rat or sheep IgG; Donkey anti-Rabbit IgG (H&L); Affinity Pure; IgG (H&L) secondary antibody
Host
Donkey
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
Purity/Purification
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Affinity purified using solid phase Rabbit IgG.
Form/Format
Lyophilized
Concentration
1 mg/ml (E 1% at 280 nm = 13.0) (varies by lot)
Applicable Applications for IgG (H&L) secondary antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
Conjugate
DyLight 350 (Ex = 353 nm; Em = 432 nm)
Immunogen
Purified Rabbit IgG, whole molecule
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% (w/v) Sodium Azide
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Country of Origin
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Preparation and Storage
Store freeze-dried powder at 2-8 degree C.
Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
Test Data
Test Data
SDS-PAGE
SDS-PAGE
Immunoelectrophoresis
Immunoelectrophoresis
Product Categories/Family for IgG (H&L) secondary antibody
Similar Products
Product Notes
The IgG (H&L) (Catalog #AAA688397) is a Secondary Antibody produced from Donkey and is intended for research purposes only. The product is available for immediate purchase. The Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG reacts with Rabbit. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (H&L) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM). Researchers should empirically determine the suitability of the IgG (H&L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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