Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human G6PC Polyclonal Antibody | anti-G6PC antibody
G6PC Antibody (Center)
IHC-P~~1:50-100
FC~~1:10-50
Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of G6PC Antibody (Center) in K562 cell line lysates (35ug/lane). G6PC (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of G6PC Antibody (Center) in K562 cell line lysates (35ug/lane). G6PC (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(G6PC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(G6PC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(G6PC Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(G6PC Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Samuel, V.T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12121-12126(2009)
Hu, C., et al. Diabetologia 52(3):451-456(2009)
NCBI and Uniprot Product Information
NCBI Description
Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]
Uniprot Description
G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.
Protein type: Transporter, SLC family; Phosphatase (non-protein); Membrane protein, integral; Endoplasmic reticulum; Transporter; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose; Membrane protein, multi-pass; Carbohydrate Metabolism - galactose; EC 3.1.3.9
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: glucose-6-phosphatase activity; phosphate binding; phosphotransferase activity, alcohol group as acceptor
Biological Process: response to food; steroid metabolic process; glycogen metabolic process; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; triacylglycerol metabolic process; urate metabolic process; regulation of gene expression; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; transmembrane transport
Disease: Glycogen Storage Disease Ia
Research Articles on G6PC
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Product Notes
The G6PC g6pc (Catalog #AAA9208384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 123-149. The G6PC Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's G6PC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)-Paraffin, Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50-100 FC~~1:10-50. Researchers should empirically determine the suitability of the G6PC g6pc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G6PC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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