Rabbit anti-Human, Mouse PLD6 Polyclonal Antibody | anti-PLD6 antibody

PLD6 Antibody (Center)

Cat. Num. AAA9204473

• Gene Names: PLD6; ZUC
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: PLD6; Polyclonal Antibody; PLD6 Antibody (Center); Mitochondrial cardiolipin hydrolase; 31--; Choline phosphatase 6; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D6; PLD 6; Protein zucchini homolog; anti-PLD6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PLD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human PLD6.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-PLD6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Region: 125-154
• Cellular Location: Mitochondrion outer membrane; Single-pass membrane protein
• Tissue Location: Widely expressed. Expression is not limited to testis and ovary.
• Function: Endonuclease that plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. piRNAs provide essential protection against the activity of mobile genetic elements (By similarity). piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface(By similarity). Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low. In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity. Produces 5' phosphate and 3' hydroxyltermini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Also acts as a regulator of mitochondrial shape through facilitating mitochondrial fusion.
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

( Western blot analysis of lysates from human ovary and mouse testis tissue lysates (from left to right), using PLD6 Antibody (Center)(Cat# MBS9204473). MBS9204473 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

( PLD6 Antibody (Center) (Cat# MBS9204473) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)

Product Categories/Family for anti-PLD6 antibody

Crown Antibodies; Microbiology; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 270265812
• NCBI GeneID: 201164
• NCBI Accession #: NP_849158.1
• NCBI GenBank Nucleotide #: NM_178836.3
• UniProt Accession #: Q8N2A8; Q8N5Y1
• NCBI Official Full Name: mitochondrial cardiolipin hydrolase
• NCBI Official Synonym Full Names: phospholipase D family, member 6
• NCBI Official Symbol: PLD6
• NCBI Official Synonym Symbols: ZUC
• NCBI Protein Information: mitochondrial cardiolipin hydrolase
• UniProt Protein Name: Mitochondrial cardiolipin hydrolase
• Protein Family: Mitochondrial cardiolipin hydrolase
• UniProt Gene Name: PLD6
• UniProt Synonym Gene Names: MitoPLD; PLD 6
• UniProt Entry Name: PLD6_HUMAN

Uniprot Description

PLD6: Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.

Protein type: Phospholipase; Membrane protein, integral; EC 3.1.4.4; EC 3.1.4.-

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrial outer membrane; integral to membrane

Molecular Function: protein homodimerization activity; metal ion binding; endonuclease activity

Biological Process: meiosis; polar granule organization and biogenesis; mitochondrial fusion; phosphatidylglycerol biosynthetic process; phospholipid metabolic process; DNA methylation during gametogenesis; glycerophospholipid biosynthetic process; gene expression; spermatid development; lipid catabolic process