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Application
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10000 results for " Antibodies to Goat Proteins" - showing 1-50
Immunohistochemistry (IHC)/Immunofluorescence (IF)
(Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations F-H. Co-expression of spike detected by spike antibodies (MBS150780) and envelope proteins detected by envelope antibodies (MBS150849) of SARS-CoV-2 (panel F) documented localization of each protein to glandular cells with negative squamous cells two weeks after full revoery (panel G, signal yellow). No signal was seen in oral swabs of positive cases (Panel H). Both the spike and envelope protein detected by anti-spike antibodies (MBS150780) and anti-envelope antibodies (MBS150849) produced a signal in the nasopharyngeal swabs of the three vases and no signal was evident in the nasopharyngeal swabs of the seven controls.)
COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA150849)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody
Reactivity
Virus
Applications
IF, IHC
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of rabbit skeletal muscle lysate using MBS600655 (1:2000).)
a-Dystroglycan, Monoclonal Antibody (Cat# AAA600655)
Full Name
a-Dystroglycan (Cranin)
Reactivity
Rabbit
Applications
WB
Purity
Ascites
Pricing
$750/0.2 mL | $3,360/5x0.2 mL
Immunofluorescence (IF)
(Firure 1. Immunofluorescent Validation of MBS150780 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, MBS150780. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)
COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA150780)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody
Reactivity
Virus
Applications
IF, IHC, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot
(SARS-CoV-2(COVID-19) Nucleoprotein Antibody [3863])
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154642)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation with HumanRecombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, 35-710, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ?g/mL Lane 2: 1?g/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154641)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, MBS154639, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1ug/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154639)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Westen Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, 35-712, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ?g/mL Lane 2: 1?g/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154643)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of humannucleocapsid recombinant protein.Antibodies: SARS-CoV-2 nucleocapsid, 35-580, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2ug/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154640)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Albumin, Polyclonal Antibody (Cat# AAA539276)
Full Name
Human Albumin antibody (Alk Phos)
Gene Names
ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
Reactivity
Human
Applications
EIA, ICC, IHC, WB
Purity
Human Albumin antibody (Alk Phos) was purified by affinity chromatography.
Pricing
$525/1 mg | $2,360/5x1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Intracellular staining of the mouse fibroblast cell line L929 with MBS633258 (filled histogram) or with isotype control (open histogram).)
Actin, alpha, Smooth Muscle, Monoclonal Antibody (Cat# AAA633258)
Full Name
Actin, alpha, Smooth Muscle (PE)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Bovine, Canine, Chicken, Frog, Goat, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography
Purified by Protein G affinity chromatography
Pricing
$800/100 Tests | $3,590/5x100 Tests
Western Blot (WB)
(All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilutionLane 1: U-87 MG whole cell lysateLane 2: U-2OS whole cell lysate Lane 3: SH-SY5Y whole cell lysateLane 4: 293T/17 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: Mouse brain lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA9211211)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, IF
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Peroxidase Conjugated, Secondary Antibody (Cat# AAA198064)
Full Name
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Peroxidase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$260/1 mg | $1,140/5x1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of BACELoading: 15 ug of lysates per lane. Antibodies: BACE (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A-C: human brain tis)
BACE, Polyclonal Antibody (Cat# AAA150291)
Full Name
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
EIA, ICC, IF, IHC-P, WB
Purity
BACE Antibody is Ion exchange chromatography purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Alkaline Phosphatase Conjugated, Secondary Antibody (Cat# AAA198063)
Full Name
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Alkaline Phosphatase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$260/1 mg | $1,140/5x1 mg
Western Blot (WB)
(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
KRAS, Polyclonal Antibody (Cat# AAA9210256)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, EIA, IF, FC/FACS
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP )
CXCR4, Polyclonal Antibody (Cat# AAA151360)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IP, IF, IHC-p, FC
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using FOXN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
FOXN3, Antibody (Cat# AAA9135025)
Full Name
FOXN3 Polyclonal Antibody
Gene Names
FOXN3; CHES1; PRO1635; C14orf116
Reactivity
Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
G6PC, Polyclonal Antibody (Cat# AAA9208384)
Full Name
G6PC Antibody (Center)
Gene Names
G6PC; G6PT; GSD1; G6PC1; GSD1a
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
PCM-1, Polyclonal Antibody (Cat# AAA9209983)
Full Name
PCM-1 Antibody (Center)
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.)
GFP, Antibody (Cat# AAA534795)
Full Name
GFP antibody (biotin)
Reactivity
Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein (Aequorea victoria) Serum. no reaction was observed against Human, Mouse, and Rat ser
Pricing
$1,080/1 mg | $4,855/5x1 mg
Hepatitis C Virus (HCV) Core Antigen (a.a. 1-120), Polyclonal Antibody (Cat# AAA315074)
Full Name
Goat anti HCV Core
Applications
EIA/ELISA, Western Blot
Purity
>95% pure. Sodium sulfate precipitation & ion-exchange chromatography
Pricing
$595/1 mL | $2,525/5x1 mL
Western Blot (WB)
(Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452))
Vimentin, Monoclonal Antibody (Cat# AAA438228)
Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 and 3T3/NIH CellsLoading: 15 μg of lysates per lane.Antibodies: 3041 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PUMA, Polyclonal Antibody (Cat# AAA151208)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Test Data
IgG (H&L), Secondary Antibody (Cat# AAA688397)
Full Name
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG
Reactivity
Rabbit.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Applications
FC/FACS, IF, EIA, IM
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Rabbit IgG.
Affinity purified using solid phase Rabbit IgG.
Pricing
$290/1 mg | $1,145/5x1 mg
Western Blot (WB)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ITA6, Monoclonal Antibody (Cat# AAA9200194)
Full Name
ITA6 Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IF, EIA, WB, FC/FACS, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P2RY12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
P2RY12, Polyclonal Antibody (Cat# AAA9135576)
Full Name
P2RY12 Polyclonal Antibody
Gene Names
P2RY12; HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Goat anti Human IgM (mu chain) (Alk Phos), Secondary Antibody (Cat# AAA538880)
Full Name
Goat anti Human IgM (mu chain) (Alk Phos)
Reactivity
No reactivity is observed to non-immunoglobulin Human serum proteins or light chains on all Human immunoglobulins.
Applications
User optimized
Purity
Affinity purified using solid phase Human IgM
Pricing
$270/1 mg | $1,210/5x1 mg
Western Blot (WB)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SLC10A1 antigen affinity purified polyclonal antibody (MBS177905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( MBS176460 ) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA177905)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
FC/FACS, IF, IHC-P, IHC-F, ICC, WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Goat anti Human IgG, Heavy and Light Chain Specific Antibody, Alkaline Phosphatase Conjugated, Secondary Antibody (Cat# AAA198062)
Full Name
Goat anti Human IgG, Heavy and Light Chain Specific Antibody, Alkaline Phosphatase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$245/1 mg
Testing Data)
HNE (Hydroxynonenal), Antibody (Cat# AAA390041)
Full Name
Goat Anti-HNE (Hydroxynonenal) Affinity Pure
Applications
EIA, IB
Purity
Polyclonal antibody against HNE-KLH purified by HNE modified Portein Sepharose affinity column.
Pricing
$390/0.2 mg | $695/0.5 mg | $1,135/1 mg | $5,045/5x1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data (TD)
( All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
BDNF, Polyclonal Antibody (Cat# AAA9211799)
Full Name
BDNF Antibody (C-term)
Gene Names
BDNF; ANON2; BULN2
Reactivity
Human
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Applications
WB, IHC-P, IF, FC, E
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
( All lanes : Anti-ROR1 Antibody at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: -MB-231 whole cell lysateLane 3: NCI-H226 whole cell lysateLane 4: A549 whole cell lysateLane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ROR1, Polyclonal Antibody (Cat# AAA9200685)
Full Name
ROR1 Antibody
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 CellsLoading: 15 μg of lysates per lane.Antibodies: 3043 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
PUMA, Polyclonal Antibody (Cat# AAA150888)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
LGR5 (GPR49), Polyclonal Antibody (Cat# AAA9205024)
Full Name
LGR5 (GPR49) Antibody (Center)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
FC/FACS, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilutionLane 1: mouse thymus lysatesLane 2: rat spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOCS1, Polyclonal Antibody (Cat# AAA9210758)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from human placenta tissue lysate,RD cell line (from left to right), using ACTA1/alpha-actin Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
ACTA1/alpha-actin, Polyclonal Antibody (Cat# AAA9231831)
Full Name
ACTA1/alpha-actin Antibody (C-term)
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Predicted: Xenopus, Bovine, Chicken, Mouse, Rabbit, Rat, Pig
Applications
WB, IHC-P, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry
(MBS9217260 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
beta Tubulin, Polyclonal Antibody (Cat# AAA9217260)
Full Name
beta Tubulin
Gene Names
Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
Reactivity
Mouse, Rat; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Applications
WB, EIA, FC, IHC-P
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Anti-LGR5/GPR49 Antibody (N-term) at 1:1000 dilution + Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
LGR5/GPR49, Polyclonal Antibody (Cat# AAA9214227)
Full Name
LGR5/GPR49 Antibody (N-term)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Mouse (Predicted: Human)
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Human pancreas tissues and Human kidney tissues, using TTR Antibody (C-term). MBS9200982 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
TTR, Polyclonal Antibody (Cat# AAA9200982)
Full Name
TTR Antibody (C-term)
Gene Names
TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651
Reactivity
Human, Rat
Applications
WB, EIA, IF, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
PLD6, Polyclonal Antibody (Cat# AAA9204473)
Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
WB, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-Activin A Receptor Type IB(ACVR1B) Antibody (N-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Activin A Receptor Type IB (ACVR1B), Polyclonal Antibody (Cat# AAA9201918)
Full Name
Activin A Receptor Type IB (ACVR1B) Antibody (N-term)
Gene Names
ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
Reactivity
Human, Mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
S100B, Polyclonal Antibody (Cat# AAA9212013)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
WB, IHC-P-Leica, FC/FACS, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
CD45, Monoclonal Antibody (Cat# AAA245401)
Full Name
Mouse Monoclonal [clone 3G4] (IgG2a) to Human CD45
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Protein G Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
IgG, Fc, X-Adsorbed, Polyclonal Antibody (Cat# AAA615214)
Full Name
IgG, Fc, X-Adsorbed (AP)
Reactivity
Rabbit
Applications
EL/EIA, WB, IHC
Purity
Purified by immunoaffinity chromatography.
Pricing
$705/1 mg | $3,165/5x1 mg
Testing Data
(Overlay histogram showing Ramos cells stained with MBS9203899 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9203899, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
BACH2, Polyclonal Antibody (Cat# AAA9203899)
Full Name
BACH2 Antibody (C-term)
Gene Names
BACH2; BTBD25
Reactivity
Human
Applications
FC, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemical (IHC)
(Immunohistochemical analysis ofparaffin-embedded human kidney tissue using MBS9202710 performed on the Leica® BOND RXm.Samples were incubated with primary antibody(1/500) for 1 hours at room temperature.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
ALPL, Polyclonal Antibody (Cat# AAA9202710)
Full Name
ALPL Antibody (Center)
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, mouse
Applications
FC, EIA, IHC-P-Leica, WB, IF
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
