Rabbit LGR5 (GPR49) Polyclonal Antibody | anti-LGR5 antibody

LGR5 (GPR49) Antibody (Center)

Cat. Num. AAA9205024

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LGR5 (GPR49); Polyclonal Antibody; LGR5 (GPR49) Antibody (Center); Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; LGR5; GPR49; GPR67; anti-LGR5 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 443-473
• Sequence Length: 883

• Applicable Applications for anti-LGR5 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000; IHC~~1:100; IHC-P~~1:10~50; FC~~1:10~50
• Calculated MW: 99998 kDa
• Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
• Cellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven.
• Tissue location: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LGR5 (GPR49) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry

(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1(MBS9205024). MBS9205024 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DABstaining.)

Western Blot

(Western blot analysis of anti-LGR5/GPR49Antibody (Center) (MBS9205024) in K562cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.)

Western Blot

(LGR5/GPR49 Antibody (Center)(MBS9205024) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).)

Related Product Information for anti-LGR5 antibody

LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.

Product Categories/Family for anti-LGR5 antibody

Cancer; Signal Transduction; GPCR Antibodies

References

Barker,N., Nature 449 (7165), 1003-1007 (2007) McClanahan,T., Cancer Biol. Ther. 5 (4), 419-426 (2006) Yamamoto,Y., Hepatology 37 (3), 528-533 (2003) Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)

NCBI and Uniprot Product Information

• NCBI GI #: 469608414
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264155.1; NP_003658.1
• NCBI GenBank Nucleotide #: NM_001277226.1
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67
• UniProt Entry Name: LGR5_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inner ear development