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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " Antibodies to Goat Proteins" - showing 100-150
Test Data
(Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FOXG1, Polyclonal Antibody (Cat# AAA9205338)
Full Name
FOXG1 Antibody (Center)
Gene Names
FOXG1; BF1; BF2; QIN; FKH2; HBF2; HFK1; HFK2; HFK3; KHL2; FHKL3; FKHL1; FKHL2; FKHL3; FKHL4; HBF-1; HBF-2; HBF-3; FOXG1A; FOXG1B; FOXG1C; HBF-G2
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat, Xenopus, Drosophila)
Applications
WB, EIA, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(A dominant 59.6 kDa band and faint 87.6 kDa band for both the Hela wild type and knock out lysates were observed (3 ug/ml anti-PSEN1) vs the predicted size of 52.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. The presence of bands in the knock out lysates suggest incomplete knockout of the target gene.)
Presenilin 1, Polyclonal Antibody (Cat# AAA9232251)
Full Name
Presenilin 1 Antibody (C-term) [Knockout Validated]
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Human, Mouse
Predicted: Rat, Monkey
Predicted: Rat, Monkey
Applications
WB, IHC, IF, FC/FACS
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005608)
Full Name
VPS35 Antibody, Clone 7E4: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$520/0.1 mg | $2,135/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TIPARP antibody (MBS9134334) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
TIPARP, Polyclonal Antibody (Cat# AAA9134334)
Full Name
TIPARP Polyclonal Antibody
Gene Names
TIPARP; PARP7; ARTD14; pART14
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Mouse liver, using IL6ST MBS9134654 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IL6ST, Polyclonal Antibody (Cat# AAA9134654)
Full Name
IL6ST Polyclonal Antibody
Gene Names
IL6ST; CD130; GP130; CDW130; IL-6RB
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-HMMR Antibody (C-term) at 1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: LNCaP whole cell lysatesLane 3: HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
HMMR, Polyclonal Antibody (Cat# AAA9214366)
Full Name
HMMR Antibody (C-term)
Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human
Applications
WB, IHC-P, FC, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data(TD)
(All lanes : Anti-ATG7 Antibody (C-term) at1:500 dilution Lane 1: Caki-1 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HepG2whole cell lysate Lysates/proteins at 40 µg perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 78 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
ATG7, Polyclonal Antibody (Cat# AAA9204169)
Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808790)
Full Name
Methylglyoxal Antibody, Clone 9E7
Applications
WB, ICC, IF, IHC, FC
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PTP4A2 using anti-PTP4A2 antibody (MBS177865).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Skeletal Muscle Tissue Lysate,Lane 2: Rat Thymus Tissue Lysate,Lane 3: Mouse Brain Tissue Lysate,Lane 4: Mouse Thymus Tissue Lysate,Lane 5: 22RV1 Whole Cell Lysate,Lane 6: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP4A2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTP4A2 at approximately 19KD. The expected band size for PTP4A2 is at 19KD. )
PTP4A2, Polyclonal Antibody (Cat# AAA177865)
Full Name
Anti-PTP4A2 Antibody
Gene Names
PTP4A2; HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808803)
Full Name
Methylglyoxal Antibody, Clone 9E7: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808763)
Full Name
Malondialdehyde Antibody, Clone 6H6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TONSL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
TONSL, Polyclonal Antibody (Cat# AAA9133084)
Full Name
TONSL Polyclonal Antibody
Gene Names
TONSL; IKBR; NFKBIL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Nephrin (Y1210), Polyclonal Antibody (Cat# AAA9208203)
Full Name
Nephrin (Y1210) antibody
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MC1R antibody (MBS9133200) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
MC1R, Polyclonal Antibody (Cat# AAA9133200)
Full Name
MC1R Polyclonal Antibody
Gene Names
MC1R; CMM5; MSH-R; SHEP2
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse spleen, using MS4A7 antibody (MBS9135481) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
MS4A7, Polyclonal Antibody (Cat# AAA9135481)
Full Name
MS4A7 Polyclonal Antibody
Gene Names
MS4A7; CFFM4; MS4A8; 4SPAN2; CD20L4
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DDC Antibody (N-term)(MBS9201818) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
DDC, Polyclonal Antibody (Cat# AAA9201818)
Full Name
DDC Antibody (N-term)
Gene Names
DDC; AADC
Reactivity
Human, mouse
Applications
EIA, IHC-P, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-BCORL1 Antibody (N-term) at 1:500 dilution + HL-60 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
BCORL1, Polyclonal Antibody (Cat# AAA9213215)
Full Name
BCORL1 Antibody (N-term)
Gene Names
BCORL1; BCoR-L1; CXorf10
Reactivity
Human
Applications
FC/FACS, EIA, WB, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of Mouse kidney, using RNASE6 antibody (MBS9135298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
RNASE6, Polyclonal Antibody (Cat# AAA9135298)
Full Name
RNASE6 Polyclonal Antibody
Gene Names
RNASE6; RAD1; RNS6; RNasek6
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-CCK4 (PTK7) Antibody (N-term)at 1:1000-1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Caco2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CCK4 (PTK7), Polyclonal Antibody (Cat# AAA9203896)
Full Name
CCK4 (PTK7) Antibody (N-term)
Gene Names
PTK7; CCK4; CCK-4
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center). MBS9204638 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Ubiquilin1, Polyclonal Antibody (Cat# AAA9204638)
Full Name
Ubiquilin1 Antibody (Center)
Gene Names
UBQLN1; DA41; DSK2; UBQN; XDRP1; PLIC-1
Reactivity
Reactivity: Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
EIA, IHC-P, WB, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808589)
Full Name
Acrolein Antibody, Clone 2H2: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclophilin A using anti- Cyclophilin A antibody (MBS1750616).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cyclophilin A antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin A at approximately 18KD. The expected band size for Cyclophilin A is at 18KD. )
Cyclophilin A, Polyclonal Antibody (Cat# AAA1750616)
Full Name
Anti-Cyclophilin A Picoband Antibody
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPN13 antibody (MBS9133056) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
PTPN13, Polyclonal Antibody (Cat# AAA9133056)
Full Name
PTPN13 Polyclonal Antibody
Gene Names
PTPN13; PNP1; FAP-1; PTP1E; PTPL1; PTPLE; PTP-BL; hPTP1E; PTP-BAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Western Blot (WB)
(All lanes : Anti-LPAR6 Antibody (Center) at 1:2000 dilution Lane 1: Molt-4 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse heart tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LPAR6, Polyclonal Antibody (Cat# AAA9212496)
Full Name
LPAR6 Antibody (Center)
Gene Names
LPAR6; LAH3; P2Y5; ARWH1; HYPT8; P2RY5
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EZH2, Polyclonal Antibody (Cat# AAA9202800)
Full Name
EZH2 Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of MPZ Antibody (C-term) in CEM cell line lysates (35ug/lane). MPZ (arrow) was detected using the purified Pab.)
MPZ, Polyclonal Antibody (Cat# AAA9207336)
Full Name
MPZ Antibody (C-term)
Gene Names
MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
Reactivity
Human
Applications
EIA, IHC-p, WB, FC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005634)
Full Name
VPS35 Antibody, Clone 5A9: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$540/0.1 mg | $2,185/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BAK1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
BAK1, Polyclonal Antibody (Cat# AAA125424)
Full Name
BAK1 Polyclonal Antibody
Gene Names
BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using HNRNPH2 antibody (MBS9133045) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
HNRNPH2, Polyclonal Antibody (Cat# AAA9133045)
Full Name
HNRNPH2 Polyclonal Antibody
Gene Names
HNRNPH2; FTP3; MRXSB; NRPH2; HNRPH'; HNRPH2; hnRNPH'
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K79 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
H3K79me3, Antibody (Cat# AAA126215)
Full Name
Histone H3K79me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using Phospho-NF2-S518 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
NF2-S518, Antibody (Cat# AAA128450)
Full Name
Phospho-NF2-S518 Polyclonal Antibody
Gene Names
NF2; ACN; SCH; BANF
Applications
WB, IF
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808698)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FAM208A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
FAM208A, Polyclonal Antibody (Cat# AAA9133235)
Full Name
FAM208A Polyclonal Antibody
Gene Names
FAM208A; TASOR; RAP140; se89-1; C3orf63
Reactivity
Human
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(TMEM100 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TMEM100 antibody detected the TMEM100 protein (arrow).)
TMEM100, Polyclonal Antibody (Cat# AAA9212673)
Full Name
TMEM100 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NMNAT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
NMNAT2, Polyclonal Antibody (Cat# AAA9133850)
Full Name
NMNAT2 Polyclonal Antibody
Gene Names
NMNAT2; PNAT2; C1orf15
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GCC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
GCC2, Polyclonal Antibody (Cat# AAA9133831)
Full Name
GCC2 Polyclonal Antibody
Gene Names
GCC2; REN53; GCC185; RANBP2L4
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Anti-CSF1R Antibody at 1:2000 dilution +HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
CSF1R, Polyclonal Antibody (Cat# AAA9203531)
Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
IF, WB, FC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808601)
Full Name
Acrolein Antibody, Clone 10A10: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using LPCAT4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
LPCAT4, Polyclonal Antibody (Cat# AAA9134321)
Full Name
LPCAT4 Polyclonal Antibody
Gene Names
LPCAT4; AYTL3; AGPAT7; LPEAT2; LPAAT-eta
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KIF22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
KIF22, Polyclonal Antibody (Cat# AAA9135271)
Full Name
KIF22 Polyclonal Antibody
Gene Names
KIF22; KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot
( Anti-AGL Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 175 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
AGL, Polyclonal Antibody (Cat# AAA9201231)
Full Name
AGL Antibody (C-term)
Gene Names
AGL; GDE
Reactivity
Human
Applications
WB, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NTN4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NTN4, Polyclonal Antibody (Cat# AAA9133795)
Full Name
NTN4 Polyclonal Antibody
Gene Names
NTN4; PRO3091
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-WNT5A Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: PANC-1 whole cell lysate Lane 3: mouse heart lysate Lane 4: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WNT5A, Polyclonal Antibody (Cat# AAA9210555)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
EIA, IHC-P, IHC-P-Leica, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(ALOX12B Antibody (C-term) (MBS9201052) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
ALOX12B, Polyclonal Antibody (Cat# AAA9201052)
Full Name
ALOX12B Antibody (C-term)
Gene Names
ALOX12B; ARCI2; 12R-LOX
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of IFNB1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.)
IFNB1, Polyclonal Antibody (Cat# AAA9211658)
Full Name
IFNB1 Antibody (N-term)
Gene Names
IFNB1; IFB; IFF; IFNB
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC-p, FC
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescent
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized mouse skin tissue labeling Pdx1 with MBS9204466 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).)
Srms, Polyclonal Antibody (Cat# AAA9204466)
Full Name
Mouse Srms Antibody (Center)
Gene Names
Srms; srm; A230069J08Rik
Reactivity
Mouse
Applications
IF, WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SLC14A2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
SLC14A2, Polyclonal Antibody (Cat# AAA9133256)
Full Name
SLC14A2 Polyclonal Antibody
Gene Names
SLC14A2; UT2; UTA; UTR; HUT2; UT-A2; hUT-A6
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NGF antibody (MBS9134225) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s)
NGF, Polyclonal Antibody (Cat# AAA9134225)
Full Name
NGF Polyclonal Antibody
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Reactivity
Human, Mouse
Applications
WB, IF, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysate from MAD-MB-231 cell line, using USP17 Antibody (C-term). MBS9211987 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
USP17, Polyclonal Antibody (Cat# AAA9211987)
Full Name
USP17 Antibody (C-term)
Reactivity
Human
Applications
WB,FC,ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
