Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 CellsLoading: 15 μg of lysates per lane.Antibodies: 3043 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
Rabbit anti-Human PUMA Polyclonal Antibody | anti-BBC3 antibody
PUMA Antibody
Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 CellsLoading: 15 μg of lysates per lane.Antibodies: 3043 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 CellsLoading: 15 μg of lysates per lane.Antibodies: 3043 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 μg of lysates per lane.Antibodies: 3041 (3 μg/mL), 3043 (2 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 μg of lysates per lane.Antibodies: 3041 (3 μg/mL), 3043 (2 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Immunofluorescence (IF)
(Figure 3 Immunofluorescence Validation of PUMAImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with 3043 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 3 Immunofluorescence Validation of PUMAImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with 3043 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).)
Immunohistochemistry (IHC)
(Figure 4 Immunohistochemistry Validation of PUMAImmunohistochemistry analysis of 4% paraformaldehyde-fixed human breast carcinoma cells labeling PUMA with 3043 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution. Image showing cytosol staining in the cancer cells.)
Immunohistochemistry (IHC)
(Figure 4 Immunohistochemistry Validation of PUMAImmunohistochemistry analysis of 4% paraformaldehyde-fixed human breast carcinoma cells labeling PUMA with 3043 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution. Image showing cytosol staining in the cancer cells.)
Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of PUMAImmunohistochemical analysis of 4% paraformaldehyde-fixed human breast tissue labeling PUMA with 3043 at 2.5 μg/ml, followed by goat anti-rabbit IgG secondary antibody at 1/250 dilution. Image showing both cytosol staining on K562 cells.)
Immunohistochemistry (IHC)
(Figure 5 Immunohistochemistry Validation of PUMAImmunohistochemical analysis of 4% paraformaldehyde-fixed human breast tissue labeling PUMA with 3043 at 2.5 μg/ml, followed by goat anti-rabbit IgG secondary antibody at 1/250 dilution. Image showing both cytosol staining on K562 cells.)
Immunofluorescence (IF)
(Figure 6 Immunofluorescence Validation of PUMAImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with 3043 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells)
Immunofluorescence (IF)
(Figure 6 Immunofluorescence Validation of PUMAImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with 3043 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells)
Western Blot (WB)
(Figure 7 KO Validation of PUMA (Michalak et al., 2008)Western blot analysis ofthymocytes from wt, noxa knockout, puma knockout and noxa/puma double knockout mice cultured for 7 h in the presence or absence of 2.5 Gy g-irradiation.PUMA expression was not detected in puma KO and double KO mice with anti-PUMA antibodies (3043).)
Western Blot (WB)
(Figure 7 KO Validation of PUMA (Michalak et al., 2008)Western blot analysis ofthymocytes from wt, noxa knockout, puma knockout and noxa/puma double knockout mice cultured for 7 h in the presence or absence of 2.5 Gy g-irradiation.PUMA expression was not detected in puma KO and double KO mice with anti-PUMA antibodies (3043).)
Western Blot (WB)
(Figure 8 KO Validation of PUMA (Ambacher et al., 2012)Puma expression is induced by potassium withdrawal in cerebellar granule neurons. After 7 days in culture CGNs were either maintained in media containing 25 mM potassium (K25) or switched to low potassium medium containing 5 mM potassium (K5). PUMA protein levels were analyzed by western blot with anti-PUMA antibodies (3043). PUMA expression was not detected in PUMA KO mice and was increased after treatment in WT.)
Western Blot (WB)
(Figure 8 KO Validation of PUMA (Ambacher et al., 2012)Puma expression is induced by potassium withdrawal in cerebellar granule neurons. After 7 days in culture CGNs were either maintained in media containing 25 mM potassium (K25) or switched to low potassium medium containing 5 mM potassium (K5). PUMA protein levels were analyzed by western blot with anti-PUMA antibodies (3043). PUMA expression was not detected in PUMA KO mice and was increased after treatment in WT.)
Western Blot (WB)
(Figure 9 Induced Expression of PUMA in MCF7 cells (Wade et al., 2008)Western analysis of MCF7 treated with the indicated dose of Nutlin-3a orABT-737 for 24h. Note that Puma is induced following Nutlin-3a treatment in these cells and PUMA expression was detected by anti-PUMA antibodies (3043))
Western Blot (WB)
(Figure 9 Induced Expression of PUMA in MCF7 cells (Wade et al., 2008)Western analysis of MCF7 treated with the indicated dose of Nutlin-3a orABT-737 for 24h. Note that Puma is induced following Nutlin-3a treatment in these cells and PUMA expression was detected by anti-PUMA antibodies (3043))
Immunofluorescence (IF)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
Immunofluorescence (IF)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
Uniprot Description
BBC3B: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity. Induced by DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. {ECO:0000269|PubMed:11463392}. Isoform 3 does not interact with BCL2. 4 isoforms of the human protein are produced by alternative splicing
Protein type: Apoptosis; Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol
Molecular Function: protein binding
Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
Research Articles on BBC3
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Product Notes
The BBC3 bbc3 (Catalog #AAA150888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PUMA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PUMA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). PUMA antibody can be used for detection of PUMA by Western blot at 2 mug/mL. Antibody can also detect PUMA by immunohistochemistry at 10 mug/mL. For immunofluorescence start at 10 mug/mL. Knockdown (Knockout, KO validated) Antibody. Researchers should empirically determine the suitability of the BBC3 bbc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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