Western Blot (WB)
(Western Blot analysis of rabbit skeletal muscle lysate using MBS600655 (1:2000).)
Mouse anti-Rabbit a-Dystroglycan Monoclonal Antibody
a-Dystroglycan (Cranin)
Reactivity
Rabbit
Applications
Western Blot
Purity
Ascites
Synonyms
a-Dystroglycan; Monoclonal Antibody; a-Dystroglycan (Cranin); Anti -a-Dystroglycan (Cranin); anti-a-Dystroglycan antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4A145
Specificity
Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig.
Purity/Purification
Ascites
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-a-Dystroglycan antibody
Inhibition, Western Blot (WB)
Application Notes
Inhibition of Laminin Binding to Dystroglycan:In anitrocellulose overlay experiment, this antibody inhibits bindingof 125I Laminin to Dystroglycan.Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan inrabbit skeletal muscle. Rabbit skeletal muscle lysate wasresolved by electrophoresis, transferred to nitrocellulose andprobed with D9905-05. Proteins were visualized using an IgG(HRP) goat anti-mouse and a chemiluminescence detectionsystem.Note:The use of WGA purified protein results in significantlycleaner blots and immunoprecipitates. Post-translationalmodification of Dystroglycan causes band broadening. Optimal dilutions to be determined by the researcher. Other applications not tested.
Immunogen
Rabbit skeletal muscle membrane preparation.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of rabbit skeletal muscle lysate using MBS600655 (1:2000).)
Western Blot (WB)
(Western Blot analysis of rabbit skeletal muscle lysate using MBS600655 (1:2000).)
Related Product Information for anti-a-Dystroglycan antibody
Dystroglycans are essential elements of the neuromuscularjunction (NMJ). The gene for dystroglycan is expressed as aprecursor protein that is post translationally cleaved into a156kD extracellular peripheral membrane protein called alphadystroglycan and a 43kD transmembrane protein, betadystroglycan. The latter protein contains a PPxY motif thatpromotes binding to WW domain containing proteins, such asutrophin and dystrophin. Phosphorylation at tyrosine 892within the PPxY motif may regulate c-Src interactions with betadystroglycan, as well as inhibit interactions with WW domainproteins. In skeletal muscle, beta dystroglycan is normallylocalized to the plasma membrane, however phosphorylationof Tyr892 leads to localization of beta dystroglycan toendosomal compartments along with c-Src. Thus,phosphorylation at Tyr892 may have important roles in alteringthe localization of beta dystroglycan during NMJ formation
Product Categories/Family for anti-a-Dystroglycan antibody
Similar Products
Product Notes
The a-Dystroglycan (Catalog #AAA600655) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The a-Dystroglycan (Cranin) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's a-Dystroglycan can be used in a range of immunoassay formats including, but not limited to, Inhibition, Western Blot (WB). Inhibition of Laminin Binding to Dystroglycan:In anitrocellulose overlay experiment, this antibody inhibits bindingof 125I Laminin to Dystroglycan.Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan inrabbit skeletal muscle. Rabbit skeletal muscle lysate wasresolved by electrophoresis, transferred to nitrocellulose andprobed with D9905-05. Proteins were visualized using an IgG(HRP) goat anti-mouse and a chemiluminescence detectionsystem.Note:The use of WGA purified protein results in significantlycleaner blots and immunoprecipitates. Post-translationalmodification of Dystroglycan causes band broadening. Optimal dilutions to be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the a-Dystroglycan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a-Dystroglycan, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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