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Application
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163 results for "Costimulation" - showing 1-50
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-PD-1 antibody RMP1-14 Staining of murine splenocytes (unstimulated and 3-day Con A stimulated) with the rabbit-chimeric version of RMP1-14 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody. Con A stimulation induces PD-1 expression as evident by the increased fluorescence signal.)
PD-1, Monoclonal Recombinant Antibody (Cat# AAA488363)
Full Name
Anti-PD-1 [RMP1-14]
Reactivity
Mouse
Applications
BL, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213057)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Dog, Guinea Pig
Applications
FC/FACS, IP
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD40 antibody G28.5 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of G28.5 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD40, Recombinant Antibody (Cat# AAA488086)
Full Name
Anti-CD40 [G28.5]
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Applications
FC/FACS, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213058)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA213845)
Full Name
RAT ANTI HUMAN CD18:RPE
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
ELISA (EIA)
( Binding curve of anti-B7-H3 antibody MJ18 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
B7-H3, Monoclonal Recombinant Antibody (Cat# AAA488371)
Full Name
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
( Binding curve of anti-ICOSL antibody HK5.3 to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
ICOSL, Monoclonal Recombinant Antibody (Cat# AAA488367)
Full Name
Anti-ICOSL [HK5.3]
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR, Monoclonal Recombinant Antibody (Cat# AAA488333)
Full Name
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
4-1BB, Monoclonal Recombinant Antibody (Cat# AAA488379)
Full Name
Anti-4-1BB [LOB12.3]
Gene Names
Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-GITRL (Tumor necrosis factor ligand superfamily member 18) antibody YGL386 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGL386 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
GITRL, Monoclonal Recombinant Antibody (Cat# AAA488331)
Full Name
Anti-GITRL [YGL386]
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Mouse
Applications
FC/FACS, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA219819)
Full Name
RAT ANTI HUMAN CD18:Low Endotoxin
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, FN, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
PD-1, Monoclonal Recombinant Antibody (Cat# AAA488364)
Full Name
Anti-PD-1 [RMP1-14]
Reactivity
Mouse
Applications
BL, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(MBS9600688 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
LCP1, Polyclonal Antibody (Cat# AAA9600688)
Full Name
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
ELISA (EIA)
( Binding curve of anti-PDL2 antibody TY25 to mouse PDL2-Fc fusion protein ELISA Plate coated with mouse PDL2-Fc fusion protein (Pr00156-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
PD-L2, Monoclonal Recombinant Antibody (Cat# AAA488369)
Full Name
Anti-PD-L2 [TY25]
Gene Names
Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
Reactivity
Mouse
Applications
FC/FACS, IP, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD27, Monoclonal Recombinant Antibody (Cat# AAA488376)
Full Name
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
FC/FACS, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-CD27 antibody on CD27-Fc fusion protein, with a CD70 (CD27-L) competition assay. Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 and isotype control (anti-Fluorescein antibody and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein was also performed (yellow line). Inhibition of the binding to Pr00160-1.9 (CD27) by (CD70) may be observed between 10,000 ng/mL and 123 ng/mL, whereas does not inhibit a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488375)
Full Name
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
FC/FACS, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD40, Recombinant Antibody (Cat# AAA488085)
Full Name
Anti-CD40 [G28.5]
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Applications
FC/FACS, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
PD-L2, Monoclonal Recombinant Antibody (Cat# AAA488370)
Full Name
Anti-PD-L2 [TY25]
Gene Names
Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
Reactivity
Mouse
Applications
FC/FACS, IP, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
B7-H3, Monoclonal Recombinant Antibody (Cat# AAA488372)
Full Name
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ICOSL, Monoclonal Recombinant Antibody (Cat# AAA488368)
Full Name
Anti-ICOSL [HK5.3]
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
GITRL, Monoclonal Recombinant Antibody (Cat# AAA488332)
Full Name
Anti-GITRL [YGL386]
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Mouse
Applications
FC/FACS, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 (MBS488471; red line) and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using MBS488471. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for MBS488471 on Pr00163-1.9.)
TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488471)
Full Name
Anti-TIGIT [4D4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
4-1BB, Monoclonal Recombinant Antibody (Cat# AAA488380)
Full Name
Anti-4-1BB [LOB12.3]
Gene Names
Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
GITR, Monoclonal Recombinant Antibody (Cat# AAA488334)
Full Name
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA211045)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$415/100 Tests | $1,710/5x100 Tests
ELISA (EIA)
(ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 (MBS488469; red line) and isotype control to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein (Pr00129-1.28) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using MBS488469. For signal detection, a 1:4000 dilution of HRP-fused anti-rabit IgG antibody was used.)
4-1BBL, Monoclonal Recombinant Antibody (Cat# AAA488469)
Full Name
Anti-4-1BBL [TKS-1]
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
TL1A, Monoclonal Recombinant Antibody (Cat# AAA488477)
Full Name
Anti-TL1A [TAN2-2]
Gene Names
Tnfsf15; Tl1; Tl1a; Vegi; Tnlg1b
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot analysis of Hela cell lysate, using LCP1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
LCP1, Polyclonal Antibody (Cat# AAA9608038)
Full Name
LCP1 Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Recombinant Antibody (Cat# AAA488480)
Full Name
Anti-Tim-4 [5G3]
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Surface plasmon resonance (SPR) sensorgram anti-TIM-1 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein (Pr00227-1.9) to immbolised the rabbit IgG chimeric version of 3B3. Pr00227-1.9 was injected and allowed to associate with between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. Pr00227-1.9 showed rapid binding to and following ligand removal remained strongly bound. This suggests that has a very high affinity for Pr00227-1.9.)
Tim-1, Monoclonal Recombinant Antibody (Cat# AAA488475)
Full Name
Anti-Tim-1 [3B3]
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
TL1A, Monoclonal Recombinant Antibody (Cat# AAA488478)
Full Name
Anti-TL1A [TAN2-2]
Gene Names
Tnfsf15; Tl1; Tl1a; Vegi; Tnlg1b
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Galectin 9, Monoclonal Recombinant Antibody (Cat# AAA488482)
Full Name
Anti-Galectin 9 [RG9-35]
Gene Names
Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
Reactivity
Mouse
Applications
FC/FACS, BL, FA
Purity
Protein A Affinity Purified
Pricing
$460/0.2 mg
Western Blot (WB)
(Western Blot using anti-CD28 antibody Rat spleen lysate was resolved on a 12% SDS PAGE gel and blots probed with at 2 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)
CD28, Monoclonal Recombinant Antibody (Cat# AAA488400)
Full Name
Anti-CD28 [D665]
Reactivity
Mouse
Applications
FC/FACS, SPR, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 and isotype control to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein (Pr00129-1.28) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-fused anti-rabit IgG antibody was used.)
4-1BBL, Monoclonal Recombinant Antibody (Cat# AAA488470)
Full Name
Anti-4-1BBL [TKS-1]
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (MBS488479) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488479, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Recombinant Antibody (Cat# AAA488479)
Full Name
Anti-Tim-4 [5G3]
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for on Pr00163-1.9.)
TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488472)
Full Name
Anti-TIGIT [4D4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-BTLA antibody on BTLA-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-BTLA antibody HMBT-6B2 and isotype control to an ELISA plate coated with BTLA-Fc fusion protein (Pr00224-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml of was used. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 3333ng/ml is suggestive of a possible secondary binding epitope for on Pr00224-1.9.)
BTLA, Monoclonal Recombinant Antibody (Cat# AAA488473)
Full Name
Anti-BTLA [HMBT-6B2]
Gene Names
Btla; A630002H24
Reactivity
Mouse
Applications
FC/FACS, WB, IP, IB, BL, FA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD40L, Monoclonal Recombinant Antibody (Cat# AAA488481)
Full Name
Anti-CD40L [MR1]
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Mouse
Applications
FC/FACS, BL, FA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (MBS488476; red line) and isotype control to an ELISA plate coated with mouse TIM-1-Fc fusion protein (Pr00227-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using MBS488476. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)
Tim-1, Monoclonal Recombinant Antibody (Cat# AAA488476)
Full Name
Anti-Tim-1 [3B3]
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD28 antibody Rat thymus (lane 1), rat lymph node (lane 2), rat thyroid (lane 3) and rat spleen (lane 4) sample were resolved on a 12% SDS PAGE gel and blots probed at 3 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)
CD28, Monoclonal Recombinant Antibody (Cat# AAA488399)
Full Name
Anti-CD28 [JJ316]
Gene Names
Cd28; CD28RNA
Reactivity
Rat
Applications
FC/FACS, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD28 antibody Rat thymus (lane 1), rat lymph node (lane 2), rat thyroid (lane 3) and rat spleen (lane 4) sample were resolved on a 12% SDS PAGE gel and blots probed at 3 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)
CD28, Monoclonal Recombinant Antibody (Cat# AAA488398)
Full Name
Anti-CD28 [JJ316]
Gene Names
Cd28; CD28RNA
Reactivity
Rat
Applications
FC/FACS, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-BTLA antibody on BTLA-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-BTLA antibody HMBT-6B2 and isotype control to an ELISA plate coated with BTLA-Fc fusion protein (Pr00224-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml of MBS488474 was used. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 3333ng/ml is suggestive of a possible secondary binding epitope for MBS488474 on Pr00224-1.9.)
BTLA, Monoclonal Recombinant Antibody (Cat# AAA488474)
Full Name
Anti-BTLA [HMBT-6B2]
Gene Names
Btla; A630002H24
Reactivity
Mouse
Applications
FC/FACS, WB, IP, IB, BL, FA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD28 antibody Rat spleen lysate was resolved on a 12% SDS PAGE gel and blots probed with at 2 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)
CD28, Monoclonal Recombinant Antibody (Cat# AAA488401)
Full Name
Anti-CD28 [D665]
Reactivity
Mouse
Applications
FC/FACS, SPR, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD86/B7-2, Monoclonal Antibody (Cat# AAA673454)
Full Name
Rat Anti-Mouse CD86/B7-2-PE
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$280/0.1 mg | $1,110/5x0.1 mg
CD103 (Integrin alpha IEL), Monoclonal Antibody (Cat# AAA673586)
Full Name
Hamster Anti-Mouse CD103-FITC
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$400/0.5 mg | $1,645/5x0.5 mg
Testing Data
CD103 (Integrin alpha IEL), Monoclonal Antibody (Cat# AAA673587)
Full Name
Hamster Anti-Mouse CD103-BIOT
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$370/0.5 mg | $1,520/5x0.5 mg
CD103 (Integrin alpha IEL), Monoclonal Antibody (Cat# AAA673589)
Full Name
Hamster Anti-Mouse CD103-LE/AF
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$365/0.5 mg | $1,495/5x0.5 mg
CD106 (VCAM-1), Monoclonal Antibody (Cat# AAA674161)
Full Name
Mouse Anti-Human CD106/VCAM-1-PE
Reactivity
Human
Applications
Frozen Sections
Pricing
$290/100 Tests | $1,155/5x100 Tests
CD103 (Integrin alpha IEL), Monoclonal Antibody (Cat# AAA673585)
Full Name
Hamster Anti-Mouse CD103-UNLB
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$355/0.5 mg | $1,440/5x0.5 mg
CD103 (Integrin alpha IEL), Monoclonal Antibody (Cat# AAA673588)
Full Name
Hamster Anti-Mouse CD103-PE
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$275/0.1 mg | $1,085/5x0.1 mg
