TL1A recombinant antibody

Anti-TL1A [TAN2-2]

Cat. Num. AAA488477

• Gene Names: Tnfsf15; Tl1; Tl1a; Vegi; Tnlg1b
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Protein A Affinity Purified
• Synonyms: TL1A; Monoclonal Recombinant Antibody; Anti-TL1A [TAN2-2]; Tnfsf15; bM20K13.3 (tumor necrosis factor (ligand) superfamily; member 15); MGC129934; MGC129935; TL1; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; tumor necrosis factor (ligand) superfamily member 15; tumor necrosis factor (ligand) superfamily; member 15; tumor necrosis factor ligand 1B; Tumor necrosis factor ligand superfamily member 15; Vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A; TL1A recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: TAN2-2
• Specificity: This antibody is specific for TL1A, a member of the TNF ligand family. TL1A is expressed by endothelial cells, tissue macrophages, monocyte-derived dendritic cells, plasma cells and lamina propria-derived lymphocytes. Among peripheral blood cells, TL1A has been shown to be expressed predominantly by CD4+CCR9+ lymphocytes. Its expression has been shown to be induced by TNF alpha, IL-1 alpha and IFN gamma.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 270

• Applicable Applications for TL1A recombinant antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Blocking (BL), Therapeutic
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Rat
• Immunogen: This antibody was raised by immunising rats with a soluble recombinant TL1A (T77-L252):Fc protein.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: This antibody has been used in FACS analyses to reveal the role of TL1A in humoral immune responses and its mechanism of action in the development of collagen-induced arthritis (Wang et al, 2013), and in immunofluorescence confocal microscopy to demonstrate how chronic DR3 signaling could promote pathogenesis in inflammatory bowel disease (Taraban et al, 2011). The therapeutic potential of this antagonistic anti-TL1A antibody in countering the DR3–TL1A pathway in the inflammatory joint disease has been confirmed in both of the murine adjuvant-induced arthritis (AIA) and collagen-induced arthritis (CIA) models (Bull et al, 2008). In addition, this antibody has been demonstrated to prevent chronic colitis and attenuate established colitis by down-regulation of both T-helper 1 and T-helper 17 activation (Takedatsu et al, 2008).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for TL1A recombinant antibody

Recombinant monoclonal antibody to TL1A. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma TAN2-2.

Product Categories/Family for TL1A recombinant antibody

Immunology; Costimulation; Immune-checkpoint; Cell Signalling; T Cells

References

Bull Melanie et al. The Death Receptor 3–TNF-like protein 1A pathway drives adverse bone pathology in inflammatory arthritis. J Exp Med. 2008 Oct 27;205(11):2457-64. PMID:18824582
Original Published Journal: J Exp Med. 2008 Oct 27;205(11):2457-64.
Publication Note: Describe the original generation of this antibody and its applications in two murine models of arthritis to demonstrate that the Death Receptor 3-TNF-like protein 1A (DR3-TL1A) pathway drives adverse bone pathology in inflammatory arthritis and represents a potential novel target for therapeutic intervention in inflammatory joint disease.

NCBI and Uniprot Product Information

• NCBI GI #: 238776819
• NCBI GeneID: 326623
• NCBI Accession #: NP_796345.3
• NCBI GenBank Nucleotide #: NP_796345.3
• UniProt Accession #: Q5UBV8
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 15
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 15
• NCBI Official Symbol: Tnfsf15
• NCBI Official Synonym Symbols: Tl1; Tl1a; Vegi; Tnlg1b
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 15
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 15
• UniProt Gene Name: Tnfsf15
• UniProt Synonym Gene Names: Tl1; Vegi
• UniProt Entry Name: TNF15_MOUSE

Uniprot Description

TNFSF15: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine; Apoptosis

Cellular Component: extracellular space; membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: caspase activation; cytokine metabolic process; apoptosis; activation of NF-kappaB-inducing kinase; immune response; signal transduction; positive regulation of cytokine secretion