Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR recombinant antibody
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
GITR; Monoclonal Recombinant Antibody; Anti-GITR [YGITR765]; Tumor necrosis factor receptor superfamily member 18; TNFRSF18; AITR; CD357; GITR-D; activation-inducible TNFR family receptor; glucocorticoid-induced tumor necrosis factor receptor; GITR recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
YGITR765
Specificity
This antibody binds murine GITR.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
234
Applicable Applications for GITR recombinant antibody
Flow Cytometry (FC/FACS), Block (BL)
Application Notes
A chimeric version of this antibody featuring a human IgG1 Fc-portion engineered to lack the N-linked glycosylation site at amino acid 297 was shown to have an agonistic effect on GITR signalling.
Immunogen
nB2/6TG cells transfected with mouse GITR injected into DA rats.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for GITR recombinant antibody
Recombinant monoclonal antibody to GITR. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YGITR765.
nB2/6TG cells transfected with mouse GITR injected into DA rats.
nB2/6TG cells transfected with mouse GITR injected into DA rats.
Product Categories/Family for GITR recombinant antibody
References
Cobbold et al. Induction of foxP3+ regulatory T cells in the periphery of T cell receptor transgenic mice tolerized to transplants. J Immunol. 2004 May 15; 172(10):6003-10. PMID:15128783
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GITR (Catalog #AAA488333) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-GITR [YGITR765] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GITR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Block (BL). A chimeric version of this antibody featuring a human IgG1 Fc-portion engineered to lack the N-linked glycosylation site at amino acid 297 was shown to have an agonistic effect on GITR signalling. Researchers should empirically determine the suitability of the GITR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GITR, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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