CD27 recombinant antibody
Anti-CD27 [RM27-3E5]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
Neutralization Assay, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Protein A Affinity Purified
Synonyms
CD27; Monoclonal Recombinant Antibody; Anti-CD27 [RM27-3E5]; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; CD27 recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, Kappa
Clone Number
RM27-3E5
Specificity
This antibody is specific for the extracellular domain of murine CD27, a TNF-receptor superfamily member.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
250
Applicable Applications for CD27 recombinant antibody
Neutralization, Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
This antibody recognises murine CD27, as confirmed by flow cytometry and immunoprecipitation analysis, a member of the TNF-receptor superfamily. The interaction between CD27 and its ligand CD70, which is recognised by anti-CD70 antibody TAN 1-7, is crucial to regulation of the survival and differentiation of T cells, B cells and NK cells. As a result, RM27-3E5 modulates multiple immune cell types. RM27-3E5 does not deplete T cells, NKT or NK cells, but does enhance the proliferation of anti-CD3-stimulated splenic T cells. In murine models of melanoma, it promotes the persistence of tumour-specific CD8+ T cells within tumours, and reduces levels of PD-1 expression on CD8+ T cells. Additionally, anti-CD27 reduces the proportion of FoxP3+ CD4+ T cells in tumours, and enhances the effector activity of IFNgamma-secreting, tumour-infiltrating CD8+ T cells and NK cells. Finally, anti-CD27 Ab treatment alters murine splenocyte cytokine production, significantly reducing IL-5 and IFN-gamma synthesis, and increasing TNF-alpha production. It also significantly suppresses total serum IgE levels, while significantly increasing IgG1 and IgG2a levels. These immune modulatory properties translate into potent anti-tumour effects in mice. Treatment of established murine melanoma with RM27-3E5 resulted in a significant reduction in lung metastases and subcutaneous tumours, through an IFNgamma-dependent mechanism mediated by CD8+ T cells and NK cells. In murine models of T cell lymphoma, the tumour-specific CTL response induced by RM27-3E5 promoted almost complete regression of tumours, regardless of CD27 expression on tumour cells. This antibody has been used to identify CD27-expressing cells through flow cytometry.
Immunogen
This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for CD27 recombinant antibody
Recombinant monoclonal antibody to CD27. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RM27-3E5.
This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
Product Categories/Family for CD27 recombinant antibody
References
Sumi et al. CD27 and CD70 do not play a critical role in the development of experimental allergic conjunctivitis in mice Immunology Letters, Volume 119, Issues 1-2, 15 August 2008, Pages 91-96 PMID:18579220
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,164 Da
NCBI Official Full Name
CD27 antigen isoform a
NCBI Official Synonym Full Names
CD27 antigen
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
S152; Tp55; Tnfrsf7
NCBI Protein Information
CD27 antigen
UniProt Protein Name
CD27 antigen
Protein Family
UniProt Gene Name
Cd27
UniProt Synonym Gene Names
Tnfrsf7
Uniprot Description
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Research Articles on CD27
Similar Products
Product Notes
The CD27 cd27 (Catalog #AAA488376) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD27 [RM27-3E5] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, Neutralization, Flow Cytometry (FC/FACS), Immunoprecipitation (IP). This antibody recognises murine CD27, as confirmed by flow cytometry and immunoprecipitation analysis, a member of the TNF-receptor superfamily. The interaction between CD27 and its ligand CD70, which is recognised by anti-CD70 antibody TAN 1-7, is crucial to regulation of the survival and differentiation of T cells, B cells and NK cells. As a result, RM27-3E5 modulates multiple immune cell types. RM27-3E5 does not deplete T cells, NKT or NK cells, but does enhance the proliferation of anti-CD3-stimulated splenic T cells. In murine models of melanoma, it promotes the persistence of tumour-specific CD8+ T cells within tumours, and reduces levels of PD-1 expression on CD8+ T cells. Additionally, anti-CD27 reduces the proportion of FoxP3+ CD4+ T cells in tumours, and enhances the effector activity of IFNgamma-secreting, tumour-infiltrating CD8+ T cells and NK cells. Finally, anti-CD27 Ab treatment alters murine splenocyte cytokine production, significantly reducing IL-5 and IFN-gamma synthesis, and increasing TNF-alpha production. It also significantly suppresses total serum IgE levels, while significantly increasing IgG1 and IgG2a levels. These immune modulatory properties translate into potent anti-tumour effects in mice. Treatment of established murine melanoma with RM27-3E5 resulted in a significant reduction in lung metastases and subcutaneous tumours, through an IFNgamma-dependent mechanism mediated by CD8+ T cells and NK cells. In murine models of T cell lymphoma, the tumour-specific CTL response induced by RM27-3E5 promoted almost complete regression of tumours, regardless of CD27 expression on tumour cells. This antibody has been used to identify CD27-expressing cells through flow cytometry. Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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