CD40L recombinant antibody

Anti-CD40L [MR1]

Cat. Num. AAA488481

• Gene Names: Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: CD40L; Monoclonal Recombinant Antibody; Anti-CD40L [MR1]; CD154; CD40 ligand; CD40-L; Cd40l; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; RP23-153G22.3; T-BAM; T-cell antigen Gp39; TNF-related activation protein; Tnfsf5; TRAP; Tumor necrosis factor ligand superfamily member 5.; CD40L recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: MR1
• Specificity: This antibody is specific for murine CD40 ligand (CD40L), a 39 kDa transmembrane glycoprotein. CD40L is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD40L-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for CD40L recombinant antibody: Flow Cytometry (FC/FACS), Blocking (BL), Functional Assay (FA)
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Armenian Hamster
• Immunogen: This antibody was raised by immunising Armenian hamsters with murine activated Th1 (D1.6) plasma membrane as described by Noelle et al (1992).
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: This antibody has been used in various FACS analyses for diverse immunological applications, such as to indicate how naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival (Lesley et al, 2006), to prove that the interaction between natural killer cells and dendritic cells has a pivotal role in the sensitization phase of contact hypersensitivity (Shimizuhira et al, 2014), and to demonstrate that enhanced CD8 T cell responses through GITR-mediated costimulation could resolve chronic viral infection (Pascutti et al, 2015). This antibody has also been used for in vitro and in vivo blocking and functional studies, for instance, to indicate that the 39-kDa CD40L membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation (Noelle et al, 1992), and to demonstrate that the CD40L–CD40 pathway can augment the survival of autoantigen-engaged B cells in the absence of T cell activation (Lesley et al, 2006). Furthermore, this antibody clone (MR1) has been re-formatted as a rat IgG2b version and used for in vivo functional assays to suggest that short pulses of anti-CD40L antibody therapy may still be useful in tolerance protocols even when the Fc region is disabled (Daley et al, 2008).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for CD40L recombinant antibody

Recombinant monoclonal antibody to CD40L. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma MR1.

Product Categories/Family for CD40L recombinant antibody

Immunology; Costimulation; Cell Signalling; T Cells; B Cells; CD Markers

References

Noelle et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4. PMID:1378631
Original Published Journal: Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4.
Publication Note: Describe the original generation of this antibody and its subsequent use in FACS, blocking and functional studies to indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation.

NCBI and Uniprot Product Information

• NCBI GI #: 15011846
• NCBI GeneID: 21947
• NCBI Accession #: NP_035746.2
• NCBI GenBank Nucleotide #: NP_035746.2
• UniProt Accession #: P27548
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: Cd40lg
• NCBI Official Synonym Symbols: IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: Cd40lg
• UniProt Synonym Gene Names: Cd40l; Tnfsf5; CD40-L; TRAP
• UniProt Entry Name: CD40L_MOUSE

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell projection; cell surface; external side of plasma membrane; extracellular space

Molecular Function: CD40 receptor binding

Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; B cell differentiation; B cell proliferation; immunoglobulin secretion; inflammatory response; isotype switching; negative regulation of apoptosis; platelet activation; positive regulation of B cell proliferation; positive regulation of immune response to tumor cell; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-4 production; positive regulation of MHC class II biosynthetic process; positive regulation of T cell proliferation; regulation of immunoglobulin secretion