anti-Mouse CD103 (Integrin alpha IEL) Monoclonal Antibody

Hamster Anti-Mouse CD103-BIOT

Cat. Num. AAA673587

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD103 (Integrin alpha IEL); Monoclonal Antibody; Hamster Anti-Mouse CD103-BIOT; Hamster Anti-Mouse CD103 - Biotin; Hamster Anti-Mouse CD103 (Integrin alpha IEL); anti-CD103 (Integrin alpha IEL) antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG
• Clone Number: 2E7
• Specificity: The CD103/ alpha IEL beta 7 integrin complex (Mr 160 and 115 kDa peptides).; CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery.1-3 In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290.1 The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 This co-stimulatory activity is also shared by the mAb M290.4 The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.5
• Form/Format: Biotin (BIOT) Conjugate

• Applicable Applications for anti-CD103 (Integrin alpha IEL) antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): Identification and enumeration of CD103 ^+ cells by flow cytometry; Immunohistochemistry (frozen sections); In vitro activation; ; Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. LeFrancois, L., T.A. Bennett, W.L. Havran, and L. Puddington. 1994. Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells. Eur. J. Immunol. 24:635.
2. Lefrancois, L. 1991. Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal repertoire? Immunol. Today, 12(12):436.
3. Reimann, J. and Rudolphi, A. 1995. Co-expression of CD8 alpha in CD4+ T cell receptor alpha beta + T cell migration into the murine small intestine epithelial layer. Eur. J. Immunol. 25:1580.
4. Roberts, K., and P. Kilshaw. 1993. The mucosal T cell integrin alpha M290 beta 7 recognizes a ligand on mucosal epithelial cell lines. Eur. J. Immunol. 23:1630.
5. Cerf-Bensussan, N., Bengue, B., Gagnon, J. and Meo, T. 1992. Eur. J. Immunol. 22:273.