CD28 recombinant antibody

Anti-CD28 [D665]

Cat. Num. AAA488401

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protein A Affinity Purified
• Synonyms: CD28; Monoclonal Recombinant Antibody; Anti-CD28 [D665]; TP44; T-cell-specific surface glycoprotein CD28; CD28 recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: D665
• Specificity: This antibody binds to the laterally exposed C?D loop of CD28 and induces T cell proliferation without TCR ligation.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 218

• Applicable Applications for CD28 recombinant antibody: CoStim, Superagonism, Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR), Immunohistochemistry (IHC)
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Mouse
• Clone Note: This antibody binds to mouse CD28, and can trigger T-cell proliferation in vitro and in vivo in a TCR-independent manner. This antibody is a superagonistic antibody. This anitbody does not compete with E18
• Immunogen: The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Western Blot (WB)

(Western Blot using anti-CD28 antibody Rat spleen lysate was resolved on a 12% SDS PAGE gel and blots probed with at 2 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)

Related Product Information for CD28 recombinant antibody

Recombinant monoclonal antibody to CD28. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma D665.

Product Categories/Family for CD28 recombinant antibody

Immunology; Signalling; Costimulation; Superagonism; Immuno-oncology; Immunotherapy

References

Dennehy et al. Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses Journal of Immunology PMID:16670276
Original Published Journal: Journal of Immunology
Publication Note: Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses

NCBI and Uniprot Product Information

• NCBI GI #: 161484597
• NCBI GeneID: 12487
• NCBI Accession #: NP_031668.3
• NCBI GenBank Nucleotide #: NP_031668.3
• UniProt Accession #: P31041
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.