BTLA recombinant antibody

Anti-BTLA [HMBT-6B2]

Cat. Num. AAA488474

• Gene Names: Btla; A630002H24
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation, Immunoblot, Western Blot, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: BTLA; Monoclonal Recombinant Antibody; Anti-BTLA [HMBT-6B2]; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B and T lymphocyte associated protein; B- and T-lymphocyte-associated protein; BTLA1; FLJ16065; MGC129743; BTLA recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: HMBT-6B2
• Specificity: This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 306

• Applicable Applications for BTLA recombinant antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunoprecipitation (IP), Immunoblot (IB), Blocking (BL), Functional Assay (FA)
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Armenian Hamster
• Immunogen: This antibody was raised by immunising Armenian hamsters with mouse BTLA:Fc fusion protein.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: This antibody has been used in immunoblot analyses to confirm that B and T lymphocyte attenuator (BTLA) regulates T cell activation through interaction with herpesvirus entry mediator (HVEM) (Sedy et al, 2005), as well as in FACS and yeast display technology to demonstrate that BTLA exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T Cells (Hurchla et al, 2005). In a mouse conjunctivitis model, treatment with this antibody during the induction phase has been shown to decrease B-cell population, upregulate Th2 cytokine production and increase the conjunctival eosinophile numbers (Ishida et al, 2012). However, treatment with this antibody during the effector phase does not affect the development of experimental conjunctivitis (Ishida et al, 2012).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

ELISA (EIA)

(ELISA of anti-BTLA antibody on BTLA-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-BTLA antibody HMBT-6B2 and isotype control to an ELISA plate coated with BTLA-Fc fusion protein (Pr00224-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml of MBS488474 was used. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 3333ng/ml is suggestive of a possible secondary binding epitope for MBS488474 on Pr00224-1.9.)

Related Product Information for BTLA recombinant antibody

Recombinant monoclonal antibody to BTLA. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma HMBT-6B2.

Product Categories/Family for BTLA recombinant antibody

Immunology; Costimulation; Immune-checkpoint; Immuno-oncology; T Cells

References

Waka Ishida et al. B and T lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis. Graefes Arch Clin Exp Ophthalmol. 2012 Feb;250(2):289-95. PMID:21779950
Original Published Journal: Graefes Arch Clin Exp Ophthalmol. 2012 Feb;250(2):289-95.
Publication Note: Describe the use of this antibody, together with the anti-HVEM antibody, to investigate the roles that B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) play in the development of antigen-induced experimental conjunctivitis (EC).

NCBI and Uniprot Product Information

• NCBI GI #: 83700227
• NCBI GeneID: 208154
• NCBI Accession #: NP_001032808.2
• NCBI GenBank Nucleotide #: NP_001032808.2
• UniProt Accession #: Q7TSA3
• NCBI Official Full Name: B- and T-lymphocyte attenuator isoform 1
• NCBI Official Synonym Full Names: B and T lymphocyte associated
• NCBI Official Symbol: Btla
• NCBI Official Synonym Symbols: A630002H24
• NCBI Protein Information: B- and T-lymphocyte attenuator
• UniProt Protein Name: B- and T-lymphocyte attenuator
• Protein Family: B- and T-lymphocyte attenuator
• UniProt Gene Name: Btla
• UniProt Entry Name: BTLA_MOUSE

Uniprot Description

BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; immune system process; negative regulation of alpha-beta T cell proliferation; immune response-regulating cell surface receptor signaling pathway; negative regulation of B cell proliferation