ELISA (EIA)
(ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for on Pr00163-1.9.)
TIGIT recombinant antibody
Anti-TIGIT [4D4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
TIGIT; Monoclonal Recombinant Antibody; Anti-TIGIT [4D4]; VSIG9; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; Washington University Cell Adhesion Molecule; WUCAM; TIGIT recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster (Armenian) IgG kappa
Clone Number
4D4
Specificity
4D4 specifically Recognizes TIGIT.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
249
Applicable Applications for TIGIT recombinant antibody
Agonist, Flow Cytometry (FC/FACS)
Antibody Type
Recombinant Antibody
Antibody Species
Armenian Hamster
Original Species Name
Armenian Hamster
Immunogen
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Clone Note
4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA (EIA)
(ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for on Pr00163-1.9.)
ELISA (EIA)
(ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. Binding curves of the rabbit chimeric IgG version of the anti-TIGIT antibody 4D4 and isotype control to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 (BioRad 5196-2504) antibody was used. The second increase in absorbance at 1000ng/ml is suggestive of a possible secondary binding epitope for on Pr00163-1.9.)
Related Product Information for TIGIT recombinant antibody
Recombinant monoclonal antibody to TIGIT. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 4D4.
Product Categories/Family for TIGIT recombinant antibody
References
Joller et al. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol. 2011 Feb 1;186(3):1338-42 PMID:21199897
Original Published Journal: J Immunol. 2011 Feb 1;186(3):1338-42
Publication Note: Describes the generation of anti-TIGIT monoclonal antibodies and their use to analyse the function of TIGIT in cells.
Original Published Journal: J Immunol. 2011 Feb 1;186(3):1338-42
Publication Note: Describes the generation of anti-TIGIT monoclonal antibodies and their use to analyse the function of TIGIT in cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
Tigit
NCBI Official Synonym Symbols
Vstm3
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
Protein Family
UniProt Gene Name
Tigit
Uniprot Description
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Research Articles on TIGIT
Similar Products
Product Notes
The TIGIT tigit (Catalog #AAA488472) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIGIT [4D4] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIGIT can be used in a range of immunoassay formats including, but not limited to, Agonist, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the TIGIT tigit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGIT, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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