Mouse anti-Human CD106 (VCAM-1) Monoclonal Antibody
Mouse Anti-Human CD106/VCAM-1-PE
Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Synonyms
CD106 (VCAM-1); Monoclonal Antibody; Mouse Anti-Human CD106/VCAM-1-PE; Mouse Anti-Human CD106/VCAM-1 - Phycoerythrin-Labeled; Mouse Anti-Human CD106 (VCAM-1); anti-CD106 (VCAM-1) antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
1.G11B1
Specificity
Human CD106/VCAM-1
CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM- 1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokineactivated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha 4 beta 1 (CD49d/CD29, VLA-4) and alpha 4 beta 7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta 2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.1-4
CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM- 1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokineactivated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha 4 beta 1 (CD49d/CD29, VLA-4) and alpha 4 beta 7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta 2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.1-4
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for anti-CD106 (VCAM-1) antibody
ELISA; Flow Cytometry; Immunohistochemistry (Frozen Sections); Western Blot
Application Notes
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of phosphate buffered saline containing 0.1% PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD106 Section, Academic Press, New York, p. 386.
3. Thornhill, M.H., S.M. Wellicome, D.L. Mahiouz, J.S. Lanchbury, U. Kyan-Aung, and D.O. Haskard. 1991. J. Immunol. 146:592.
4. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD106 Section, Academic Press, New York, p. 386.
3. Thornhill, M.H., S.M. Wellicome, D.L. Mahiouz, J.S. Lanchbury, U. Kyan-Aung, and D.O. Haskard. 1991. J. Immunol. 146:592.
4. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767.
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Product Notes
The CD106 (VCAM-1) (Catalog #AAA674161) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD106/VCAM-1-PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD106 (VCAM-1) can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunohistochemistry (Frozen Sections); Western Blot. Flow Cytometry Suggested Dilution: 10 uL/10^6 cells. Researchers should empirically determine the suitability of the CD106 (VCAM-1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD106 (VCAM-1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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