CD28 recombinant antibody

Anti-CD28 [JJ316]

Cat. Num. AAA488398

• Gene Names: Cd28; CD28RNA
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Protein A Affinity Purified
• Synonyms: CD28; Monoclonal Recombinant Antibody; Anti-CD28 [JJ316]; TP44; T-cell-specific surface glycoprotein CD28; CD28 recombinant antibody

• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: JJ316
• Specificity: Recognizes rat CD28 and can trigger T-cell proliferation in vitro and in vivo.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 218

• Applicable Applications for CD28 recombinant antibody: CoStim, Superagonism, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Antibody Type: Recombinant Antibody
• Antibody Species: Mouse
• Original Species Name: Mouse
• Immunogen: Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.
• Clone Note: This antibody binds rat CD28 and can convey as a co-stimulatory signal. At 100-1000-fold higher concentration than required for co-stimulation JJ316 (unlike the clone JJ319 marketed by competitors (see Tacke et al. 1997)) can induce proliferation of all T-cell subsets without TCR engagement.
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Western Blot (WB)

(Western Blot using anti-CD28 antibody Rat thymus (lane 1), rat lymph node (lane 2), rat thyroid (lane 3) and rat spleen (lane 4) sample were resolved on a 12% SDS PAGE gel and blots probed at 3 ug/ml before being detected by a secondary antibody. The expected band size at 25 kDa was observed.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of rat spleen using anti-CD28 antibody Formalin fixed rat spleen slices were were stained at 3 ug/ml.)

Related Product Information for CD28 recombinant antibody

Recombinant monoclonal antibody to CD28. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma JJ316.

Product Categories/Family for CD28 recombinant antibody

Immunology; Signalling; Costimulation; Immuno-oncology; Immunotherapy

References

Tacke et al. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol PMID:9022025
Original Published Journal: Eur J Immunol
Publication Note: Describes the in vivo and in vitro characterisation of JJ316.

NCBI and Uniprot Product Information

• NCBI GI #: 6978625
• NCBI GeneID: 25660
• NCBI Accession #: NP_037253.1
• NCBI GenBank Nucleotide #: NP_037253.1
• UniProt Accession #: P31042
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: Cd28 molecule
• NCBI Official Symbol: Cd28
• NCBI Official Synonym Symbols: CD28RNA
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28
• UniProt Entry Name: CD28_RAT

NCBI Description

costimulatory molecule involved in induction of T cell proliferation and initiation of the Th2 response; may be important in maintanence of basal IgE production; binds to B7-1 or B7-2 on antigen presenting cells (APCs) [RGD, Feb 2006]

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.