Immunofluorescence (IF)
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Rat anti-Mouse Ly-6C Monoclonal Antibody | anti-Ly-6C antibody
Rat Anti Mouse Ly-6C: FITC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Immunofluorescence (IF)
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Immunofluorescence (IF)
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Testing Data
(Rat anti Mouse Ly6C antibody, clone ER-MP20 used for the evaluation of Ly-6C expression on inflammatory macrophages in a brain injury model by flow cytometry.Image caption:Dual targeting of CCR2/5 with CVC mitigates TBI-induced macrophage recruitment. a In WT mice, CVC or vehicle was administered BID via oral gavage at 100 mg/kg at 2 and 10 h post surgery before animals were euthanized for various endpoints at 24 h following surgery. b A cohort of WT aged TBI animals (n?=?8/group) was used for flow cytometry analysis of macrophage infiltration into the injured brain. Twenty-four hours after injury, there was a significant decrease in the number of peripheral macrophages (CD11b+F4/80+CD45hiLy6C+) in the CVC-treated animals compared to their vehicle-treated counterpartsFrom: Morganti JM, Riparip LK, Chou A, Liu S, Gupta N, Rosi S.Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.J Neuroinflammation. 2016 Apr 18;13(1):80.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti Mouse Ly6C antibody, clone ER-MP20 used for the evaluation of Ly-6C expression on inflammatory macrophages in a brain injury model by flow cytometry.Image caption:Dual targeting of CCR2/5 with CVC mitigates TBI-induced macrophage recruitment. a In WT mice, CVC or vehicle was administered BID via oral gavage at 100 mg/kg at 2 and 10 h post surgery before animals were euthanized for various endpoints at 24 h following surgery. b A cohort of WT aged TBI animals (n?=?8/group) was used for flow cytometry analysis of macrophage infiltration into the injured brain. Twenty-four hours after injury, there was a significant decrease in the number of peripheral macrophages (CD11b+F4/80+CD45hiLy6C+) in the CVC-treated animals compared to their vehicle-treated counterpartsFrom: Morganti JM, Riparip LK, Chou A, Liu S, Gupta N, Rosi S.Age exacerbates the CCR2/5-mediated neuroinflammatory response to traumatic brain injury.J Neuroinflammation. 2016 Apr 18;13(1):80.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and A700 conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and Pacific Blue conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on mononuclear cells in the presence of Mouse Seroblock (BUF041A). Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat anti Mouse Ly-6C . All experiments performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A).)
Testing Data
(Figure A. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Rat anti Mouse Ly-6C . All experiments performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A).)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD8a and FITC conjugated Rat anti Mouse Ly6C . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.
Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.
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Product Notes
The Ly-6C ly6c2 (Catalog #AAA225659) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Mouse Ly-6C: FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ly-6C can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the Ly-6C ly6c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly-6C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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