Integrin beta-7 Recombinant Antibody | ITGB7 recombinant antibody

Anti-Integrin beta-7 [FIB27]

Cat. Num. AAA488444

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Protein A Affinity Purified
• Synonyms: Integrin beta-7; Monoclonal Recombinant Antibody; Anti-Integrin beta-7 [FIB27]; FIB-27; FIB 27; Fib-27; Fib27; Fib 27; fib27; fib-27; fib 27; Integrin b7; integrin b-7; Gut homing receptor beta subunit; FIB27; ITGB7 recombinant antibody

• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: FIB27
• Specificity: FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta-7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 798

• Applicable Applications for ITGB7 recombinant antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Blocking (BL)
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Rat
• Immunogen: FIB27 was prepared by immunizing rats with TK1 cells.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin-mediated cell adhesion.
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 (MBS488444) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488444, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Related Product Information for ITGB7 recombinant antibody

Recombinant monoclonal antibody to Integrin beta-7. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma FIB27.

Product Categories/Family for ITGB7 recombinant antibody

Cell Adhesion; Signal Transduction; Lymphoid Cells; Immunology; Adaptive Immunity

References

Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153:3847 1994 PMID:7523506
Original Published Journal: J. Immunol. 153:3847 1994
Original Published Journal: J. Immunol. 153:3847 1994
Publication Note: Describes the use of FIB27 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping.

NCBI and Uniprot Product Information

• NCBI GI #: 4504777
• NCBI GeneID: 3695
• NCBI Accession #: NP_000880.1
• NCBI GenBank Nucleotide #: NP_000880.1
• UniProt Accession #: P26010; P26011
• NCBI Official Full Name: integrin beta-7
• NCBI Official Synonym Full Names: integrin subunit beta 7
• NCBI Official Symbol: ITGB7
• NCBI Protein Information: integrin beta-7
• UniProt Protein Name: Integrin beta-7
• Protein Family: Integrin
• UniProt Gene Name: ITGB7

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms dimers with an alpha4 chain or an alphaE chain and plays a role in leukocyte adhesion. Dimerization with alpha4 forms a homing receptor for migration of lymphocytes to the intestinal mucosa and Peyer's patches. Dimerization with alphaE permits binding to the ligand epithelial cadherin, a calcium-dependent adhesion molecule. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

ITGB7: integrin alpha-4/beta-7 (Peyer's patches-specific homing receptor lpam-1) is expected to play a role in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM 1. Integrin alpha-e/beta-7 (HML-1) is a receptor for e-cadherin. Two alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; integrin complex; membrane; plasma membrane; receptor complex

Molecular Function: cell adhesion molecule binding; protein binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte tethering or rolling; receptor clustering; regulation of immune response