Mouse anti-Human YARS Monoclonal Antibody | anti-YARS antibody

Mouse monoclonal antibody Anti-Human YARS

• Gene Names: YARS; YRS; YTS; TYRRS; CMTDIC
• Reactivity: Human
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: YARS; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human YARS; tyrosyl-tRNA synthetase (YARS); YRS; YTS; TYRRS; CMTDIC; anti-YARS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: YAR5H08
• Form/Format: Mouse monoclonal anti-human YARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA)and 0.05% sodium azide (NaN3).
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 3117

• Applicable Applications for anti-YARS antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 0.2-2ug/ml; IP: 100-500ug/sample; FC: 0.5-2ug/sample; ICC: 2-100ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Immunogen: Recombinant
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8°C for up to one year. We recommend storing at -20 degree C for long-term storage. Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-YARS monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of YARS by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. YAR5H08-2])

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-YARS mAb. [Lot No. YAR5H08-2]Predicted molecular weight: 59 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-YARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. YAR5H08-2])

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-YARS mAb. [Lot No. YAR5H08-1])

Related Product Information for anti-YARS antibody

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 156071485
• NCBI GeneID: 8565
• NCBI Accession #: NP_003671.1
• NCBI GenBank Nucleotide #: NM_003680
• NCBI Official Full Name: Homo sapiens tyrosyl-tRNA synthetase (YARS), mRNA
• NCBI Official Synonym Full Names: tyrosyl-tRNA synthetase
• NCBI Official Symbol: YARS
• NCBI Official Synonym Symbols: YRS; YTS; TYRRS; CMTDIC
• NCBI Protein Information: tyrosine--tRNA ligase, cytoplasmic; tyrosine tRNA ligase 1, cytoplasmic; tyrosyl--tRNA ligase; tyrosyl-tRNA synthetase, cytoplasmic
• UniProt Protein Name: Tyrosine--tRNA ligase, cytoplasmic
• Protein Family: Tyrosine--tRNA ligase
• UniProt Gene Name: YARS
• UniProt Entry Name: SYYC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.1; Ligase

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: extracellular space; cytoplasm; nucleus; cytosol

Molecular Function: signal transducer activity; tyrosine-tRNA ligase activity; ATP binding; interleukin-8 receptor binding; isoleucine-tRNA ligase activity; tRNA binding

Biological Process: tyrosyl-tRNA aminoacylation; isoleucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; apoptosis; gene expression; signal transduction

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C