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Reactivity

Gene Name

Isotype

Host

Application

Clone

1205 results for "Rat IgG Isotype Control" - showing 250-300


Western Blot (WB) (Figure 1. Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (MBS177838).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mouse Skeletal Muscle Tissue Lysate,Lane 2: HELA Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 150KD. The expected band size for PPP1R12A is at 115KD. )

PPP1R12A, Polyclonal Antibody (Cat# AAA177838)

Full Name
Anti-PPP1R12A Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of SSH3BP1 using anti-SSH3BP1 antibody (MBS178018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Testis Tissue Lysate Lane 3: HELA Whole Cell Lysate Lane 4: RH35 Whole Cell Lysate Lane 5: HEPA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSH3BP1 at approximately 55KD. The expected band size for SSH3BP1 is at 55KD. )

SSH3BP1, Polyclonal Antibody (Cat# AAA178018)

Full Name
Anti-SSH3BP1 Antibody
Gene Names
ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CA2 using anti- CA2 antibody (MBS178489). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CA2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CA2 at approximately 29KD. The expected band size for CA2 is at 29KD‚ )

CA2, Polyclonal Antibody (Cat# AAA178489)

Full Name
Anti-ca2 Antibody
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescent (IF) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling HINT1 with MBS9200257 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue))

HINT1, Monoclonal Antibody (Cat# AAA9200257)

Full Name
HINT1 Antibody
Gene Names
HINT1; HINT; NMAN; PKCI-1; PRKCNH1
Reactivity
Human, mouse, rat
Applications
F, IHC-P, FC, WB,E
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Quality Control (Western blot analysis of immunized recombinant protein, using anti-BTAF1 monoclonal antibody. )

BTAF1, Monoclonal Antibody (Cat# AAA120002)

Full Name
Mouse monoclonal antibody Anti-Human BTAF1
Gene Names
BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed mouse splenocytes with anti-CD105 antibody MJ7/18 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

CD105, Monoclonal Recombinant Antibody (Cat# AAA488439)

Full Name
Anti-CD105 [MJ7/18]
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse
Applications
FC/FACS, WB, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Desmosome/Hemidesmosome, Monoclonal Recombinant Antibody (Cat# AAA488464)

Full Name
Anti-Desmosome/Hemidesmosome [F12]
Reactivity
Human
Applications
IF, IEM
Purity
Affinity Purified, Using A Recombinant Lectin Column
Pricing
$635/0.05 mg | $2,660/5x0.05 mg
Testing Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)

CD172a, Monoclonal Antibody (Cat# AAA212962)

Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, WB
Pricing
$765/1 mg | $3,290/5x1 mg
Western Blot (WB) (Western blot analysis of lysates from A549 cell line,rat brain tissue,LNCaP,PC-3 cell line (from left to right), using ADRA1D Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

ADRA1D, Polyclonal Antibody (Cat# AAA9231705)

Full Name
ADRA1D Antibody (N-term)
Gene Names
ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
Reactivity
Human, Rat
Predicted: Rabbit, Bovine, Dog, Sheep
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (All lanes : Anti-NCAM1 Antibody (C-term) at 1:1000 dilutionLane 1: HT-1080 whole cell lysatesLane 2: SW480 whole cell lysatesLane 3: human cerebellum lysatesLane 4: human brain lysatesLane 5: rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

NCAM1, Polyclonal Antibody (Cat# AAA9231981)

Full Name
NCAM1 Antibody (C-term)
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human, Rat
Predicted: Bovine, Chicken, Mouse
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC) (Figure 1. IHC analysis of Hsp70 using anti-Hsp70 antibody (M00949-2).Hsp70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Hsp70 Antibody (M00949-2) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Hsp70, Monoclonal Antibody (Cat# AAA1752034)

Full Name
Anti-Hsp70 Antibody (monoclonal, 3H5)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western Blot using anti-CD2 antibody YTH 655 Human tonsil lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 655 (MBS488385) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa, but this protein is glycosylated at several residues. MBS488385 successfully detected CD2 in human tonsil lysate.)

CD2, Monoclonal Recombinant Antibody (Cat# AAA488385)

Full Name
Anti-CD2 [YTH 655]
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). MBS9210244 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

HIST1H2AG, Polyclonal Antibody (Cat# AAA9210244)

Full Name
HIST1H2AG Antibody (Center)
Gene Names
HIST1H2AI; H2A/c; H2AFC
Reactivity
Human (Predicted Reactivity: Rat, Mouse, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila)
Applications
EIA, IHC, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.08 mL
Western Blot (WB) (All lanes : Anti-CDK5 Antibody (C-term) at 1/1000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: K562 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CDK5, Polyclonal Antibody (Cat# AAA9231930)

Full Name
CDK5 Antibody (C-term)
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, Mouse
Predicted: Bovine, Rat, Xenopus
Applications
WB, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysates from mouse NIH/3T3,CEM,Hela,HepG2 cell line (from left to right), using HIST1H2AG Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

HIST1H2AG, Polyclonal Antibody (Cat# AAA9231703)

Full Name
HIST1H2AG Antibody (Center)
Reactivity
Human, Mouse
Predicted: Rat, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC) (Figure 1. IHC analysis of ITGA2B using anti-ITGA2B antibody (MBS1750570).ITGA2B was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGA2B Antibody (MBS1750570) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

ITGA2B/Cd41, Polyclonal Antibody (Cat# AAA1750570)

Full Name
Anti-ITGA2B/Cd41 Picoband antibody
Gene Names
ITGA2B; GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of lysates from mouse NIH/3T3,Hela cell line,rat brain tissue lysate (from left to right), using Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

RPS6, Monoclonal Antibody (Cat# AAA9231656)

Full Name
RPS6 Antibody (N-term)
Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat
Predicted: Monkey
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
ELISA (EIA) (ELISA of anti-4-1BBL antibody on 4-1BBL-Fc fusion protein. Binding curves of the rabbit IgG chimeric version of the anti-4-1BBL antibody TKS-1 and isotype control to an ELISA plate coated with mouse 4-1BBL Fc-fusion protein (Pr00129-1.28) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using. For signal detection, a 1:4000 dilution of HRP-fused anti-rabit IgG antibody was used.)

4-1BBL, Monoclonal Recombinant Antibody (Cat# AAA488470)

Full Name
Anti-4-1BBL [TKS-1]
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data (Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody MBS488384 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.)

CD169, Monoclonal Recombinant Antibody (Cat# AAA488384)

Full Name
Anti-CD169 [3D6.112 (Recombinant Version)]
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Testing Data (Anti-CD11b staining of murine neutrophils. Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor, clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b) or isotype control followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control, cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive, agreeing with findings obtained using hybridoma-derived antibody.)

CD11b, Monoclonal Recombinant Antibody (Cat# AAA488382)

Full Name
Anti-CD11b [5C6 (Recombinant Version)]
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Mouse, Human
Applications
IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using anti-CD44 antibody Hermes-1 Human activated T lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-1 (MBS488445, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD44, Monoclonal Recombinant Antibody (Cat# AAA488445)

Full Name
Anti-CD44 [Hermes-1]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
Applications
BL, EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (MBS488479) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488479, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)

Tim-4, Monoclonal Recombinant Antibody (Cat# AAA488479)

Full Name
Anti-Tim-4 [5G3]
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488442)

Full Name
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
IF, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 (MBS488441) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488441, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488441)

Full Name
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
IF, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Interleukin 7 receptor antibody YIL7R323 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YIL7R323 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Interleukin 7 Receptor, Monoclonal Recombinant Antibody (Cat# AAA488433)

Full Name
Anti-Interleukin 7 Receptor [YIL7R323]
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA) (ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (MBS488476; red line) and isotype control to an ELISA plate coated with mouse TIM-1-Fc fusion protein (Pr00227-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using MBS488476. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)

Tim-1, Monoclonal Recombinant Antibody (Cat# AAA488476)

Full Name
Anti-Tim-1 [3B3]
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western blot analysis of lysates from Hela cell line,human kidney tissue,HepG2,U-87 MG,U-251 MG cell line (from left to right), using NDUFC2 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

NDUFC2, Polyclonal Antibody (Cat# AAA9231707)

Full Name
NDUFC2 Antibody (N-term)
Gene Names
NDUFC2; HLC-1; B14.5b; NADHDH2; CI-B14.5b
Reactivity
Human
Predicted: Mouse, Rat
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF) (Immunofluorescence staining of fixed mouse splenocytes with anti-CD73 antibody YDBYT 208.3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YDBYT 208.3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

CD73, Monoclonal Recombinant Antibody (Cat# AAA488417)

Full Name
Anti-CD73 [YDBYT 208.3]
Gene Names
Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
Reactivity
Mouse
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)-Formallin (Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617).)

Vinculin, Monoclonal Antibody (Cat# AAA4381609)

Full Name
Vinculin (Marker of Age-related Macular Degeneration)
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (Immunofluorescent staining of PFA-fixed HeLa cells with Endoglin / CD105 Mouse Monoclonal Antibody (ENG/1621) followed by goat anti-mouse IgG-CF488 (Green). )

Endoglin/CD105, Monoclonal Antibody (Cat# AAA4381689)

Full Name
Endoglin/CD105 (Angiogenesis Marker)
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Human, Monkey, Horse, Pig, Rat
Applications
EIA, FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
SDS-Page (SDS-PAGE Analysis Purified p120 Recombinant Mouse Monoclonal Antibody (rCTNNB1/1507). Confirmation of Purity and Integrity of Antibody.)

Catenin, beta (p120), Monoclonal Antibody (Cat# AAA4381612)

Full Name
Catenin, beta (p120)
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; NEDSDV; armadillo
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Testing Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/M˜ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜ from dectin-1-deficient mice. All bars show mean+/-95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (˜C') or the use of different cell lineages (˜L'). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

DECTIN-2, Monoclonal Antibody (Cat# AAA211248)

Full Name
RAT ANTI MOUSE DECTIN-2:Low Endotoxin
Gene Names
Clec4n; Nkcl; Clec6a; Clecsf10
Applications
FC/FACS, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data (Whole cell lysate from human HEK293 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells using MBS601404. Predicted molecular weight: 273kD)

PRP8 Pre-mRNA Processing Factor 8 Homolog, Monoclonal Antibody (Cat# AAA601404)

Full Name
PRP8 Pre-mRNA Processing Factor 8 Homolog (PRPF8, PRP8, hPRP8, PRPC8, RP13)
Gene Names
PRPF8; PRP8; RP13; HPRP8; PRPC8
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, FC/FACS, GS/EMSA
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$460/0.05 mg | $2,050/5x0.05 mg
Immunofluorescence (IF) (Human hepatocellular carcinoma cell line Hep G2 was stained with rabbit IgG-UNLB isotype control (MBS679166; left) and Rabbit Anti-Human DR5-UNLB (MBS674972; Right) followed by donkey anti rabbit IgG (H+L), Mouse/rat/Human SP ads-BIOT (MBS674966) Streptavidin-CY3 and DAPI.)

Donkey Anti-Rabbit IgG (H+L), Polyclonal Secondary Antibody (Cat# AAA674965)

Full Name
Donkey Anti-Rabbit IgG (H+L), Human/Rat/Mouse ads-HRP
Reactivity
Rabbit
Applications
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
Purity
Affinity chromatography on Rabbit IgG covalently linked to agarose.
Pricing
$210/1 mL | $795/5x1 mL

Rat anti Mouse IgG1 Kappa Light Chain, Secondary Antibody (Cat# AAA531361)

Full Name
Rat anti Mouse IgG1 Kappa Light Chain
Reactivity
Specific for mouse igG1 kappa light chain by IEP.
Applications
ELISA
Pricing
$680/1 mg | $3,050/5x1 mg

Rat IgG Isotype Control, Immunoglobulin (Cat# AAA537341)

Full Name
Rat IgG Isotype Control
Applications
EIA, FC/FACS
Purity
> 97% pure
Pricing
$275/10 mg | $1,240/5x10 mg
Testing Data (A) Mouse B16F1 cells were stained with MBS633558 (filled histogram) or APC-conjugated isotype control (open histogram).)

CCR10, Monoclonal Antibody (Cat# AAA633558)

Full Name
CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G Protein-coupled Receptor 2, GRP2) (APC)
Gene Names
CCR10; GPR2
Reactivity
Mouse, Rat
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$885/100 Tests | $3,980/5x100 Tests
Testing Data (A) Mouse bEND.3 and B) Rat PC-12 cells were stained with MBS620775 (filled histogram) or isotype control (open histogram))

Neuropilin-1, Polyclonal Antibody (Cat# AAA620775)

Full Name
Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (APC)
Gene Names
NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; DKFZp781F1414; DKFZp686A03134
Reactivity
Human
Applications
FC/FACS
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$745/100 Tests | $3,355/5x100 Tests

Rat IgG Isotype Control, Isotype Control (Cat# AAA679139)

Full Name
Rat IgG-UNLB
Applications
ELISA; Flow Cytometry
Pricing
$200/10 mg | $755/5x10 mg
Flow Cytometry (FC/FACS) (C3H/HE mouse splenocytes were stained with anti-CD45 (clone: IBL-5/25) (filled histogram) or rat IgG1 isotype control (open histogram).)

CD45, Monoclonal Antibody (Cat# AAA520149)

Full Name
Anti-Mouse CD45, Purified (Clone IBL-5/25) (rat IgG1)
Applications
IHC, acetone-fixed frozen sections, FC, IP
Purity
Purified from ascitic fluid via Protein G Chromatography
Pricing
$345/0.25 mg | $1,340/5x0.25 mg
Flow Cytometry (FC/FACS) (Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug Rat IgG2b Isotype Control (open histogram) or 0.25 ug of CD279 antibody (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Total viable cells were used for analysis.)

PD1, Monoclonal Antibody (Cat# AAA832832)

Full Name
PD1 antibody
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Applications
Functional Assay
Purity
PD1 antibody was purified by affinity chromatography.
Pricing
$500/0.1 mg | $2,235/5x0.1 mg

Rat anti Mouse IgG1 Kappa Light Chain (biotin), Secondary Antibody (Cat# AAA530673)

Full Name
Rat anti Mouse IgG1 Kappa Light Chain (biotin)
Reactivity
Bovine, Dog, Pig, Sheep
Applications
IC
Pricing
$785/1 mg | $3,515/5x1 mg
Testing Data (Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype (MBS679166; left) and Rabbit Anti-human DR5-UNLB (MBS674972; right) followed by donkey Anti-rabbit IgG (H+L) Mouse/Rat/Human SP ads-BIOT( MBS674966 streptavidin-CY3 (MBS675153) and DAPI.)

Rabbit IgG Isotype Control, Isotype Control (Cat# AAA679168)

Full Name
Rabbit IgG-PE
Applications
Flow Cytometry; ELISA; FLISA; Immunocytochemistry
Pricing
$175/100 Tests | $645/5x100 Tests
Immunoprecipitation (IP) (Immunoprecipitation of NIH/3T3 cell lysates using Immobilized Rabbit (DA1E) mAb IgG XP™ Isotype Control (Bead Conjugate) and Immobilized Akt (pan) (C67E7) Rabbit mAb (Bead Conjugate). The western blot was probed using Akt (pan) (40D4) Mouse mAb.)

Akt, pan, Monoclonal Antibody (Cat# AAA606320)

Full Name
Akt, pan (Rac PKa, PKBa) (Agarose)
Gene Names
Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
Reactivity
Human, Monkey, Mouse, Rat
Applications
IP
Purity
Purified
Purified
Pricing
$640/0.4 mL | $2,865/5x0.4 mL
Flow Cytometry (FC/FACS) (Detection of LAG-3 in mouse pplenocytes by Flow Cytometry. Mouse splenocytes were treated for 72h with 50ng/ml PMA and 500ng/ml Ca2+ Ionomycin then stained with MBS642763 or isotype control antibody, followed by Allophycocyanin- conjugated Rat IgG Secondary Antibody.)

LAG3, Monoclonal Antibody (Cat# AAA642763)

Full Name
LAG3 (Lymphocyte Activation Gene 3 Protein, LAG-3, Protein FDC, CD223, FDC)
Gene Names
LAG3; CD223
Reactivity
Mouse
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
$835/0.1 mg | $3,750/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of Jurkat cells, treated with either Calyculin A or LY294002, using MBS631986 and detected with Streptavidin-HRP.)

Akt, Monoclonal Antibody (Cat# AAA631986)

Full Name
Akt, phosphorylated (Ser473) (Rac PKa, PKBa)
Gene Names
Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
Reactivity
Bovine, Hamster, Human, Mouse, Rat0
Applications
WB, FC/FACS
Purity
Supernatant
Supernatant
Pricing
$745/0.1 mL | $3,340/5x0.1 mL

Rat IgG2b Isotype Control, Immunoglobulin (Cat# AAA539673)

Full Name
Rat IgG2b Isotype Control
Applications
EIA, FC/FACS
Pricing
$290/0.5 mg | $1,305/5x0.5 mg
Flow Cytometry (FC/FACS) (Staining of 1-day LPS stimulated human peripheral blood cells with 0.5 ug of Armenian Hamster IgG Isotype Control (FITC) (open histogram) or 0.5 ug of CCL2 antibody (FITC) (filled histogram). Cells in the monocyte gate were used for analysis.)

CCL2, Monoclonal Antibody (Cat# AAA832705)

Full Name
CCL2 antibody (FITC)
Applications
FC/FACS
Pricing
$375/0.05 mg | $1,690/5x0.05 mg
Testing Data (Jurkat cells were stained with MBS603325 (filled histogram) or isotype control antibody (open histogram), followed by PE-conjugated anti-rat antibody.)

JAK1, Monoclonal Antibody (Cat# AAA603325)

Full Name
JAK1 (Janus Kinase 1)
Gene Names
jak1; jak1
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC, ICC, FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$835/0.1 mg | $3,750/5x0.1 mg
Testing Data (Whole blood monocytes were stained with MBS606914 (filled histogram) or isotype control (open histogram).)

Tim3, Monoclonal Antibody (Cat# AAA606914)

Full Name
Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)
Gene Names
HAVCR2; TIM3; KIM-3; TIMD3; Tim-3; FLJ14428; HAVCR2
Reactivity
Human
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$845/100 Tests | $3,785/5x100 Tests
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