Rabbit GRP94/HSP90B1 Antibody | anti-HSP90B1 antibody

GRP94/HSP90B1 (Endoplasmic Reticulum Marker)

Cat. Num. AAA4381313

• Gene Names: HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• Reactivity: Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: GRP94/HSP90B1; GRP94/HSP90B1 (Endoplasmic Reticulum Marker); Glucose Regulated Protein 94; 94kDa glucose-regulated protein; Endoplasmin; Endothelial cell (HBMEC) glycoprotein (ECGP); Heat shock protein 90kDa beta member 1 (HSP90B1); Stress inducible tumor rejection antigen GP96; TRA1; Tumor rejection antigen-1 (gp96); anti-HSP90B1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
• Isotype: IgG
• Clone Number: HSP90B1/3168R
• Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 803

• Applicable Applications for anti-HSP90B1 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 0.5-1ug/million cells; Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HepG2, U20S cells. About 50% of breast tumors express grp94 protein.
• Immunogen: Recombinant full-length human HSP90B1 protein
• Cellular Localization: Cytoplasmic and Nuclear
• Hu-chromosome Location: 12q23.3
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified GRP94 Recombinant Rabbit Monoclonal (HSP90B1/3168R). Confirmation of Purity and Integrity of Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)

References

1. Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990). 2. Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 4507677
• NCBI GeneID: 7184
• NCBI Accession #: NP_003290.1
• NCBI GenBank Nucleotide #: NP_003290.1
• UniProt Accession #: P14625
• Molecular Weight: 94kDa
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90 beta family member 1
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_HUMAN

NCBI Description

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Secreted; Apoptosis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q24.2-q24.3

Cellular Component: endoplasmic reticulum membrane; focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region; cytosol; extracellular matrix; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; midbody; nucleus

Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; unfolded protein response; regulation of phosphoprotein phosphatase activity; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sequestering of calcium ion; toll-like receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of apoptosis