Western Blot (WB)
(Anti- CD22 Picoband antibody, MBS177673, Western blottingAll lanes: Anti CD22 (MBS177673) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 95KDObserved bind size: 95KD )
CD22 Polyclonal Antibody | anti-CD22 antibody
Anti-CD22 Antibody
IHC: 0.5-1ug/ml
FC: 1-3ug/1x106
Tested Species: In-house tested species with positive test results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti- CD22 Picoband antibody, MBS177673, Western blottingAll lanes: Anti CD22 (MBS177673) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 95KDObserved bind size: 95KD )
Western Blot (WB)
(Anti- CD22 Picoband antibody, MBS177673, Western blottingAll lanes: Anti CD22 (MBS177673) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 95KDObserved bind size: 95KD )
Immunohistochemistry (IHC)
(Anti- CD22 Picoband antibody, MBS177673, IHC(P)IHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti- CD22 Picoband antibody, MBS177673, IHC(P)IHC(P): Human Tonsil Tissue )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of Raji cells using anti-CD22 antibody (MBS177673).Overlay histogram showing Raji cells stained with MBS177673 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (MBS177673,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of Raji cells using anti-CD22 antibody (MBS177673).Overlay histogram showing Raji cells stained with MBS177673 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (MBS177673,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Background: CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.
NCBI and Uniprot Product Information
Uniprot Description
CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: integral to membrane; plasma membrane
Molecular Function: carbohydrate binding; protein binding
Biological Process: cell adhesion
Research Articles on CD22
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Product Notes
The CD22 cd22 (Catalog #AAA177673) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD22 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.1-0.5ug/ml IHC: 0.5-1ug/ml FC: 1-3ug/1x106 Tested Species: In-house tested species with positive test results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CD22 cd22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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