Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of 7G6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Complement Receptor 1 & 2 Recombinant Antibody
Anti-Complement Receptor 1 & 2 [7G6]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A Affinity Purified
Synonyms
Complement Receptor 1 & 2; Anti-Complement Receptor 1 & 2 [7G6]; CD21 & CD35; Complement Receptor 1 & 2 recombinant antibody
Reactivity
Mouse
Isotype
Rabbit IgG, kappa
Clone Number
7G6
Specificity
Recognizes an epitope shared by 145-150kDa and 190kDa complement receptor proteins, originally designated CR2 (CD21) and CR1 (CD35).
Purity/Purification
Protein A Affinity Purified
Applicable Applications for Complement Receptor 1 & 2 recombinant antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Immunoprecipitation (IP), Western Blot (WB), Block, CoStim
Application Notes
This antibody binds mouse complement receptors CR1 and CR2, functionally blocking CR2 leading to suppression of a normal antibody immune response (see Heyman et al. 1990, PMID: 1695671)
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Purified Mouse CR1.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of 7G6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of 7G6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody Formalin fixed rat stomach slices were were stained with at 3 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody Formalin fixed rat stomach slices were were stained with at 3 ug/ml.)
Related Product Information for Complement Receptor 1 & 2 recombinant antibody
Recombinant monoclonal antibody to Complement Receptor 1 & 2. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 7G6. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for Complement Receptor 1 & 2 recombinant antibody
References
Kinoshita et al. Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative. J Immunol. 1988 May 1; 140(9):3066-72 PMID:2966205
Similar Products
Product Notes
The Complement Receptor 1 & 2 (Catalog #AAA488171) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Complement Receptor 1 & 2 [7G6] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Complement Receptor 1 & 2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Immunoprecipitation (IP), Western Blot (WB), Block, CoStim. This antibody binds mouse complement receptors CR1 and CR2, functionally blocking CR2 leading to suppression of a normal antibody immune response (see Heyman et al. 1990, PMID: 1695671). Researchers should empirically determine the suitability of the Complement Receptor 1 & 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement Receptor 1 & 2, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
