Mouse anti-Human PAX8 Monoclonal Antibody | anti-PAX8 antibody

Mouse monoclonal antibody Anti-Human PAX8

Cat. Num. AAA120074

• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: PAX8; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human PAX8; paired box gene 8; anti-PAX8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: PAX8R1
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 2698

• Applicable Applications for anti-PAX8 antibody: Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-PAX8 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of PAX8 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. PAX8R1-3]Predicted molecular weight: 48 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-PAX8 mAb. [Lot No. PAX8R1-3]Predicted molecular weight: 48 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PAX8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. PAX8R1-3])

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX8 mAb. [Lot No. PAX8R1-3])

Related Product Information for anti-PAX8 antibody

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 33987990
• NCBI GeneID: 7849
• Molecular Weight: 31,044 Da
• NCBI Official Full Name: Homo sapiens paired box 8, mRNA
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8; paired domain gene 8
• UniProt Protein Name: Paired box protein Pax-8
• Protein Family: Paired box protein
• UniProt Gene Name: PAX8
• UniProt Entry Name: PAX8_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; thyroid-stimulating hormone receptor activity

Biological Process: regulation of apoptosis; inner ear morphogenesis; anatomical structure morphogenesis; central nervous system development; transcription, DNA-dependent; ureteric bud branching; positive regulation of transcription, DNA-dependent; thyroid gland development; mesonephros development; positive regulation of transcription from RNA polymerase II promoter; kidney development; pronephros development; urogenital system development

Disease: Hypothyroidism, Congenital, Nongoitrous, 2