Rabbit anti-Human MCSF Receptor (CSF1R) Polyclonal Antibody | anti-CSF1R antibody

MCSF Receptor (CSF1R) Antibody (C-term)

Cat. Num. AAA9211301

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MCSF Receptor (CSF1R); Polyclonal Antibody; MCSF Receptor (CSF1R) Antibody (C-term); Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; M-CSF-R; Proto-oncogene c-Fms; CD115; CSF1R; FMS; anti-CSF1R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MCSF Receptor (CSF1R) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 940-971 amino acids from the C-terminal region of human MCSF Receptor (CSF1R).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 940-971
• Sequence Length: 306

• Applicable Applications for anti-CSF1R antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-p~~1:100; FC~~1:25
• Function: Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
• Cellular Location: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Expressed in bone marrow and in differentiated blood mononuclear cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of CSF1R (arrow) using rabbit polyclonal CSF1R Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSF1R gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC)

(MCSF Receptor (CSF1R) Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(All lanes : Anti-MCSF Receptor (CSF1R) Antibody (C-term) at 1:1000 dilution Lane 1: THP-1 whole cell lysate Lane 2: rat spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(WB - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail Immunohistochemical analysis of MBS9211301 on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Flow Cytometry (FC)

(WB - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) MBS9211301 detail Overlay histogram showing HepG2 cells stained with MBS9211301(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9211301, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Related Product Information for anti-CSF1R antibody

CSF1R is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. This protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.

Product Categories/Family for anti-CSF1R antibody

Cardiovascular; Immunology; Signal Transduction

References

Follows, G.A., et al., EMBO J. 22(11):2798-2809 (2003).
Riccioni, R., et al., Leukemia 17(1):98-113 (2003).
Zhu, K., et al., Biochem. Biophys. Res. Commun. 297(5):1211-1217 (2002).
Ide, H., et al., Proc. Natl. Acad. Sci. U.S.A. 99(22):14404-14409 (2002).
Flick, M.B., et al., J. Cell. Biochem. 85(1):10-23 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 27262659
• NCBI GeneID: 1436
• NCBI Accession #: NP_005202.2
• NCBI GenBank Nucleotide #: NM_005211.3
• UniProt Accession #: P07333; Q6LDW5; Q6LDY4; Q86VW7; B5A955; D3DQG2
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• Protein Family: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: Oncoprotein; EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: macrophage colony stimulating factor receptor activity; protein homodimerization activity; cytokine binding; protein phosphatase binding; ATP binding

Biological Process: regulation of bone resorption; peptidyl-tyrosine phosphorylation; macrophage differentiation; phosphoinositide-mediated signaling; monocyte differentiation; protein amino acid autophosphorylation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; osteoclast differentiation; signal transduction; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; phosphatidylinositol metabolic process; positive regulation of protein amino acid phosphorylation; intercellular junction maintenance; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids