Mouse CD161/NK1.1 Monoclonal Antibody | anti-CD161/NK1.1 antibody
Mouse Anti Mouse CD161/NK1.1: FITC
Gene Names
Klrb1c; Nk1; Ly59; Nk-1; CD161; Ly-59; Ly55c; NK1.1; NKRP1; Nk1.2; Klrb1b; NK-RP1; Nk-1.2; Nkrp1b; Nkrp1c; ly-55c; NKRP140; AI462337; NKR-P1.9
Reactivity
MouseDoes not react with: Rat, Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A
Synonyms
CD161/NK1.1; Monoclonal Antibody; Mouse Anti Mouse CD161/NK1.1: FITC; CD161/NK1.1 antibody (PK136); Mouse anti Mouse CD161/NK1.1; anti-CD161/NK1.1 antibody
Host
Mouse
Reactivity
Mouse
Does not react with: Rat, Human
Does not react with: Rat, Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
PK136
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1 mg/ml (varies by lot)
Sequence Length
271
Applicable Applications for anti-CD161/NK1.1 antibody
Flow Cytometry (FC/FACS)
Immunogen
Spleen and bone marrow cells from CE mice.
Conjugation
FITC
Carrier Free
Yes
Fusion Partners
Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD122 and FITC conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD122 and FITC conjugated Mouse anti Mouse CD161 . All experiments performed on mouse blood gated on live, single lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD8 and RPE conjugated Mouse anti Mouse CD161 . All experiments performed on red cell lysed mouse blood gated on lymphoid cells in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Rat anti Mouse CD8 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD8 and Alexa Fluor 647 conjugated Mouse anti Mouse CD161 . All experiments performed on red cell lysed mouse blood gated on lymphoid cells in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Related Product Information for anti-CD161/NK1.1 antibody
Mouse anti Mouse CD161/NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.
In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.AE40
Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.AE40
Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
killer cell lectin-like receptor subfamily B member 1C isoform 1
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily B member 1C
NCBI Official Symbol
Klrb1c
NCBI Official Synonym Symbols
Nk1; Ly59; Nk-1; CD161; Ly-59; Ly55c; NK1.1; NKRP1; Nk1.2; Klrb1b; NK-RP1; Nk-1.2; Nkrp1b; Nkrp1c; ly-55c; NKRP140; AI462337; NKR-P1.9
NCBI Protein Information
killer cell lectin-like receptor subfamily B member 1C
UniProt Protein Name
Killer cell lectin-like receptor subfamily B member 1C
UniProt Gene Name
Klrb1c
UniProt Synonym Gene Names
Ly55c; Nkrp1c; Ly-55c; NKR-P1 40
UniProt Entry Name
KLRBC_MOUSE
