Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Mouse CAPN2 Monoclonal Antibody | anti-CAPN2 antibody
CAPN2 Antibody
Predicted: Mouse
Predicted: Mouse
FC/FACS ~~ 1:100
IHC-P ~~ 1:25
Note=Translocates to the plasma membrane
upon Ca(2+) binding
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (MBS9231851) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (MBS9231851) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (MBS9231851) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (MBS9231851) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
NCBI and Uniprot Product Information
NCBI Description
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.53
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus; chromatin; lipid raft
Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity
Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis
Research Articles on CAPN2
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Product Notes
The CAPN2 capn2 (Catalog #AAA9231851) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAPN2 Antibody reacts with Human, Rat Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAPN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry Paraffin (IHC-P), Flow Cytometry (FC/FACS), ELISA (EIA). WB ~~ 1:1000 FC/FACS ~~ 1:100 IHC-P ~~ 1:25. Researchers should empirically determine the suitability of the CAPN2 capn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPN2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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