Rabbit STMN2 Polyclonal Antibody | anti-STMN2 antibody

STMN2 Antibody (N-term)

Cat. Num. AAA9214476

• Gene Names: STMN2; SCG10; SCGN10
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: STMN2; Polyclonal Antibody; STMN2 Antibody (N-term); Stathmin-2; Superior cervical ganglion-10 protein; Protein SCG10; SCG10; SCGN10; anti-STMN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This STMN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 36-69 amino acids from the N-terminal region of human STMN2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Applicable Applications for anti-STMN2 antibody: Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000; IHC~~1:25; FC~~1:25
• Cellular Location: Cytoplasm. Cytoplasm, perinuclear region. Cell projection, growth cone Membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, axon. Golgi apparatus. Endosome. Cell projection, lamellipodium Note=Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms. Colocalized with CIB1 in neurites of developing hippocampal primary neurons (By similarity). Colocalized with CIB1 in the cell body, neuritis and growth cones of neurons. Colocalized with CIB1 to the leading edge of lamellipodia.
• Tissue Location: Neuron specific.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SH-SY5Y cells using STMN2 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9214476 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. liver section using STMN2 Antibody (N-term). MBS9214476 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from U-87 MG, SH-SY5Y cell line, human brain, mouse brain, rat brain tissue lysate (from left to right), using STMN2 Antibody (N-term). MBS9214476 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-STMN2 antibody

Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).

Product Categories/Family for anti-STMN2 antibody

Crown Antibodies; Neuroscience

References

Okazaki T.,et al.Neurobiol. Aging 16:883-894(1995).
Fujiwara T.,et al.Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 312922390
• NCBI GeneID: 11075
• NCBI Accession #: NP_001186143.1
• NCBI GenBank Nucleotide #: NM_001199214.1
• UniProt Accession #: Q93045; O14952; Q6PK68; A8K9M2; G3V110
• Molecular Weight: 20828
• NCBI Official Full Name: stathmin-2 isoform 1
• NCBI Official Synonym Full Names: stathmin 2
• NCBI Official Symbol: STMN2
• NCBI Official Synonym Symbols: SCG10; SCGN10
• NCBI Protein Information: stathmin-2
• UniProt Protein Name: Stathmin-2
• Protein Family: Stathmin
• UniProt Gene Name: STMN2
• UniProt Synonym Gene Names: SCG10; SCGN10; Protein SCG10
• UniProt Entry Name: STMN2_HUMAN

NCBI Description

This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Nov 2010]

Uniprot Description

STMN2: a cytoskeletal protein that may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Neuron specific.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: Golgi apparatus; neuron projection; growth cone; membrane; cell soma; axon; lamellipodium; perinuclear region of cytoplasm; cytoplasm; vesicle; endosome

Molecular Function: tubulin binding; protein binding; calcium-dependent protein binding

Biological Process: positive regulation of microtubule depolymerization; negative regulation of microtubule depolymerization; negative regulation of microtubule polymerization