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Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).)
Mouse Histone H1 Antibody | anti-H1 antibody
Histone H1 (Pan Nuclear Marker)
Gene Names
H1-0; H10; H1.0; H1F0; H1FV
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Histone H1; Histone H1 (Pan Nuclear Marker); His1; HisC; HIST1; HIST1H1A; HIST1H1B; HIST1H1C; HIST1H1D; HIST1H1E; HIST1H1T; Oocyte-specific histone H1; Testicular H1 histone; anti-H1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG2a, kappa
Clone Number
rAE-4
Specificity
Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a purer preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
240
Applicable Applications for anti-H1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Immunogen
Recombinant full-length human Histone H1 protein
Cellular Localization
Nuclear
Hu-chromosome Location
3q21-q22; 6q21-q22; 22q13.1
Antibody Type
Recombinant Mouse Monoclonal Antibody
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).)
SDS-Page
(SDS-PAGE Analysis Purified Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4). Confirmation of Integrity and Purity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4). Confirmation of Integrity and Purity of Antibody.)
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Immunofluorescence (IF)
(Immunofluorescent staining of HeLa cells using Histone H1 Mouse Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Phalloidin is used to label cellmembrane (red).)
Immunofluorescence (IF)
(Immunofluorescent staining of HeLa cells using Histone H1 Mouse Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Phalloidin is used to label cellmembrane (red).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~30kDa
NCBI Official Full Name
histone H1
NCBI Official Synonym Full Names
H1.0 linker histone
NCBI Official Symbol
H1-0
NCBI Official Synonym Symbols
H10; H1.0; H1F0; H1FV
NCBI Protein Information
histone H1.0
Protein Family
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]
Research Articles on H1
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Product Notes
The H1 (Catalog #AAA4380970) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H1 (Pan Nuclear Marker) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. FC/FACS: 1-2ug/million cells IF: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the H1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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