Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Rat anti-Mouse CD31 Monoclonal Antibody | anti-CD31 antibody
Rat Anti Mouse CD31: FITC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock (BUF041A).)
Testing Data
(Figure A. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock (BUF041A).)
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and Pacific Blue conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and Pacific Blue conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Testing Data
(Rat anti Mouse CD31 antibody, clone ER-MP12 used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.Image caption:Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and beta3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean +/- SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 mum.From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.The Effect of Stromal Integrin beta3-Deficiency on Two Different Tumors in Mice.Cancers (Basel). 2016 Jan 12;8(1). pii: E14.)
Testing Data
(Rat anti Mouse CD31 antibody, clone ER-MP12 used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.Image caption:Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and beta3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean +/- SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 mum.From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.The Effect of Stromal Integrin beta3-Deficiency on Two Different Tumors in Mice.Cancers (Basel). 2016 Jan 12;8(1). pii: E14.)
Testing Data
(Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)
Testing Data
(Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)
CD31 is also expressed on a major population of macrophage/dendritic cell precursors in the bone marrow. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).
NCBI and Uniprot Product Information
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: extracellular space; cell surface; smooth muscle contractile fiber; membrane; integral to membrane; plasma membrane; intracellular; cell junction; lipid raft; external side of plasma membrane
Molecular Function: protein binding
Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; wound healing; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of actin filament polymerization; positive regulation of leukocyte migration; diapedesis; cell adhesion; regulation of cell migration; phagocytosis; Rho protein signal transduction
Research Articles on CD31
Similar Products
Product Notes
The CD31 pecam1 (Catalog #AAA225658) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Mouse CD31: FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
