Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD74 recombinant antibody
Anti-CD74 [In-1]
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
CD74; Monoclonal Recombinant Antibody; Anti-CD74 [In-1]; Ia antigen; DHLAG; HLADG; Ia-gamma; H-2 class II histocompatibility antigen gamma chain; CLIP; LN-1; LN1; IN1; CD74 recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
In-1
Specificity
This antibody binds to murine CD74.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
214
Applicable Applications for CD74 recombinant antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen
Application Notes
The In-1 antibody was used to identify CD74 on lymphocytes in mice. It can be used in immunoprecipitation as well as in flow cytometry and frozen-section IHC.
Immunogen
Mouse lymphocytes of strain A injected into Wistar rats.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Western Blot (WB)
( Western Blot using anti-CD74 antibody In-1 Mouse spleen (A) and mouse lymph node (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of In-1 at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kDa, and this protein is glycosylated at several residues. successfully detected CD74 in mouse spleen and mouse lymph node lysates.)
Western Blot (WB)
( Western Blot using anti-CD74 antibody In-1 Mouse spleen (A) and mouse lymph node (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of In-1 at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kDa, and this protein is glycosylated at several residues. successfully detected CD74 in mouse spleen and mouse lymph node lysates.)
Related Product Information for CD74 recombinant antibody
Recombinant monoclonal antibody to CD74. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma In-1.
Mouse lymphocytes of strain A injected into Wistar rats.
Mouse lymphocytes of strain A injected into Wistar rats.
Product Categories/Family for CD74 recombinant antibody
References
Koch et al. Ia invariant chain detected on lymphocyte surfaces by monoclonal antibody Nature. 1982 Oct 14; 299(5884):644-5. PMID:6181417
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD74 antigen
NCBI Official Symbol
CD74
NCBI Protein Information
CD74 antigen
Protein Family
Research Articles on CD74
Similar Products
Product Notes
The CD74 (Catalog #AAA488365) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD74 [In-1] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD74 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen. The In-1 antibody was used to identify CD74 on lymphocytes in mice. It can be used in immunoprecipitation as well as in flow cytometry and frozen-section IHC. Researchers should empirically determine the suitability of the CD74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD74, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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