Mouse CD3 Monoclonal Antibody | anti-CD3E antibody

CD3 (T-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439364

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD3; Monoclonal Antibody; CD3 (T-Cell Marker) Mouse Monoclonal Antibody; CD3E; CD3 epsilon; CD3 TCR complex; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex; anti-CD3E antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG3, kappa
• Clone Number: RIV9
• Specificity: Reacts with five invariable CD3 chains (designated as and) with molecular weight ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
• Form/Format: 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 207

• Applicable Applications for anti-CD3E antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies (Order Ab without Azide); Flow Cytometry (1-2 ug/million cells); Immunofluorescence (1-2ug/ml); ; Optimal dilution for a specific application should be determined.
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: Human peripheral lymphocytes
• Hu-Chromosome Location: 11q23.3
• Positive Control: Jurkat cells. Tonsil and lymph node
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE AnalysisCD3e Mouse Monoclonal Antibody (RIV9).Confirmation of Integrity and Purity of Antibody)

Flow Cytometry (FC)

(Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9).Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red))

References

Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200 Vaessen LM et al. RIV-9: a mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7 Jiskoot W et al. Purification and stabilisation of a poorly soluble mouse IgG3 monoclonal antibody. J Immunol Methods 1991, 138(2):181-189 Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46 Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989 Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Alberola-Ila J et al. Stimulation through the TCR/CD3 complex up-regulates the CD2 surface expression on human T lymphocytes. J Immunol 1991, 146(4):108

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 20kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: external side of plasma membrane; plasma membrane; T cell receptor complex

Molecular Function: protein binding; protein heterodimerization activity; receptor signaling protein activity; SH3 domain binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of T cell proliferation; regulation of immune response; signal complex assembly; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 18