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10000 results for "Conjugated Secondary Antibodies" - showing 1-50
Western Blot (WB)
(Western blot analysis of lysates from Hela, SW480, mouse NIH/3T3 cell line and rat testis tissue lysate(from left to right), using UBE2D2 Antibody (N-term). MBS9213327 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
UBE2D2, Polyclonal Antibody (Cat# AAA9213327)
Full Name
UBE2D2 Antibody (N-term)
Gene Names
UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
Reactivity
Human, mouse
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western Blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with MBS606917 (2ug/ml) followed by HRP-conjugated anti-mouse IgG secondary antibody. A specific band was detected for ADAMTS1 at ~60-70kD (as indicated). This experiment was conducted under non-reducing conditions and using Blotting Buffer: 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20; Blocking Solution: 5% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4; Antibody Solution: 2% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4.)
ADAMTS1, Monoclonal Antibody (Cat# AAA606917)
Full Name
ADAMTS1 (A Disintegrin And Metalloproteinase with ThromboSpondin-1 motif, METH-1)
Gene Names
ADAMTS1; C3-C5; METH1; KIAA1346
Reactivity
Human
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$800/0.1 mg | $3,590/5x0.1 mg
Western Blot (WB)
(Detection of Human ADAMTS15 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2ug/ml of Human ADAMTS15 Monoclonal Antibody followed by HRP- conjugated Mouse IgG Secondary Antibody. A specific band was detected for mature ADAMTS15 at approximately 75kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
ADAMTS15, Monoclonal Antibody (Cat# AAA6001180)
Full Name
ADAMTS15 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Pricing
$735/0.1 mg | $3,290/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: HL-60 whole cell lysateLane 3: mouse testis lysateLane 4: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GMCL1, Polyclonal Antibody (Cat# AAA9216192)
Full Name
GMCL1 Antibody (Center)
Gene Names
GMCL1; GCL; GCL1; BTBD13; SPATA29
Reactivity
Human, Mouse
Applications
WB
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-ACE-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
ACE2 / ACE-2, Polyclonal Antibody (Cat# AAA243413)
Full Name
Rabbit Polyclonal to Human ACE2 / ACE-2
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
IHC - Paraffin, WB, FC/FACS
Purity
Ammonium sulfate precipitation
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
(IQCJ Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IQCJ antibody detected the IQCJ protein (arrow).)
IQCJ, Polyclonal Antibody (Cat# AAA9214546)
Full Name
IQCJ Antibody (C-term)
Reactivity
Human, mouse
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Goat anti Rabbit IgG, Fc Fragment Specific Antibody, Peroxidase Conjugated, Secondary Antibody (Cat# AAA198066)
Full Name
Goat anti Rabbit IgG, Fc Fragment Specific Antibody, Peroxidase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$295/1 mg | $1,265/5x1 mg
anti-Mouse IgG+HRP, Secondary Antibody (Cat# AAA440122)
Full Name
anti-Mouse IgG+HRP
Reactivity
Mouse
Applications
WB
Pricing
$170/0.2 mg | $705/5x0.2 mg
Western Blot (WB)
(Western blot analysis of TMPRSS2 Antibody (N-term) (MBS9215011) in 293, NCI-H460 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)
TMPRSS2, Polyclonal Antibody (Cat# AAA9215011)
Full Name
TMPRSS2 Antibody (N-term)
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human
Applications
WB, IHC-P, FC, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(STAMBPL1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the STAMBPL1 antibody detected STAMBPL1 protein (arrow).)
STAMBPL1, Polyclonal Antibody (Cat# AAA9203629)
Full Name
STAMBPL1 Antibody (Center)
Gene Names
STAMBPL1; AMSH-FP; AMSH-LP; ALMalpha; bA399O19.2
Reactivity
Human, mouse
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214794)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC, IF
Pricing
$195/0.025 mg | $730/5x0.025 mg
Western Blot (WB)
(All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilutionLane 1: U-87 MG whole cell lysateLane 2: U-2OS whole cell lysate Lane 3: SH-SY5Y whole cell lysateLane 4: 293T/17 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: Mouse brain lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA9211211)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, IF
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. MBS9204880 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Phospho-CDC25A (T507), Polyclonal Antibody (Cat# AAA9204880)
Full Name
Phospho-CDC25A (T507) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Immunohistochemistry (IHC)
(MBS9601318 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZO 1, Polyclonal Antibody (Cat# AAA9601318)
Full Name
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-SFTPC / Surfactant Protein C antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
SFTPC / Surfactant Protein C, Polyclonal Antibody (Cat# AAA249642)
Full Name
Anti-SFTPC / Surfactant Protein C Antibody (aa1-30) IHC-plus
Gene Names
SFTPC; SP-C; PSP-C; SFTP2; SMDP2; BRICD6
Reactivity
Human, Mouse
Predicted: Cow
Predicted: Cow
Applications
IHC, IF, WB
Purity
Protein A purified
Pricing
$665/0.1 mL
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Peroxidase Conjugated, Secondary Antibody (Cat# AAA198064)
Full Name
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Peroxidase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$260/1 mg | $1,140/5x1 mg
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Alkaline Phosphatase Conjugated, Secondary Antibody (Cat# AAA198063)
Full Name
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Alkaline Phosphatase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$260/1 mg | $1,140/5x1 mg
Western Blot (WB)
(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
KRAS, Polyclonal Antibody (Cat# AAA9210256)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, EIA, IF, FC/FACS
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of U251 cells, using ATG16L Antibody.U251 cells(right) were treated with Chloroquine (50 uM,16h).MBS9208353 was diluted at 1:25 dilution.Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
ATG16L, Polyclonal Antibody (Cat# AAA9208353)
Full Name
ATG16L Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot(WB)
(Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Predicted band size: 40 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.)
G6PC, Polyclonal Antibody (Cat# AAA9208384)
Full Name
G6PC Antibody (Center)
Gene Names
G6PC; G6PT; GSD1; G6PC1; GSD1a
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MUSK Antibody (MBS9205728) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
MUSK, Polyclonal Antibody (Cat# AAA9205728)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
WB, EIA, FC, IF
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
PCM-1, Polyclonal Antibody (Cat# AAA9209983)
Full Name
PCM-1 Antibody (Center)
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA212026)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Immunofluorescence (IF)
(issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.)
GFP, Antibody (Cat# AAA534795)
Full Name
GFP antibody (biotin)
Reactivity
Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein (Aequorea victoria) Serum. no reaction was observed against Human, Mouse, and Rat ser
Pricing
$1,080/1 mg | $4,855/5x1 mg
Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term). MBS9206616 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
RSPO2, Polyclonal Antibody (Cat# AAA9206616)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, IHC-P, FC, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.)
Collagen Type I, Polyclonal Antibody (Cat# AAA534202)
Full Name
Collagen Type I antibody (biotin)
Gene Names
COL1A1; OI1; OI2; OI3; OI4; EDSC
Reactivity
Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens.
Applications
EIA, IHC, IP, WB
Purity
Collagen type I antibody (biotin) was purified by affinity chromatography.
Pricing
$910/0.1 mg | $4,090/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-ACTRIIB / ACVR2B antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)
ACTRIIB / ACVR2B, Polyclonal Antibody (Cat# AAA247275)
Full Name
Rabbit Polyclonal to Human ACTRIIB / ACVR2B
Gene Names
ACVR2B; HTX4; ACTRIIB; ActR-IIB
Reactivity
Human, Mouse
Applications
IHC, IHC-P, WB
Purity
Ammonium sulfate precipitation
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
CD16/CD32, Recombinant Antibody (Cat# AAA488078)
Full Name
Anti-CD16/CD32 [2.4G2]
Gene Names
Fcgr3; CD16
Reactivity
Mouse
Applications
FC/FACS, IF, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot analysis of lysate from human kidney tissue lysate, using ACE2(SARS-R) Antibody. MBS9204203 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA9204203)
Full Name
ACE2 (SARS Receptor) Antibody (Center)
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
EIA, IHC-P, FC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ITA6, Monoclonal Antibody (Cat# AAA9200194)
Full Name
ITA6 Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IF, EIA, WB, FC/FACS, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Goat anti Human IgM (mu chain) (Alk Phos), Secondary Antibody (Cat# AAA538880)
Full Name
Goat anti Human IgM (mu chain) (Alk Phos)
Reactivity
No reactivity is observed to non-immunoglobulin Human serum proteins or light chains on all Human immunoglobulins.
Applications
User optimized
Purity
Affinity purified using solid phase Human IgM
Pricing
$270/1 mg | $1,210/5x1 mg
Western Blot (WB)
(Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody (Center). MBS9201729 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Alkaline Phosphatase (ALPI), Polyclonal Antibody (Cat# AAA9201729)
Full Name
Alkaline Phosphatase (ALPI) Antibody (Center)
Gene Names
ALPI; IAP
Reactivity
Human
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SLC10A1 antigen affinity purified polyclonal antibody (MBS177905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( MBS176460 ) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA177905)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
FC/FACS, IF, IHC-P, IHC-F, ICC, WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using MBS600002 (3ug/ml) overnight at 4°C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA600002)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
IHC, ICC, FC/FACS
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
$835/0.1 mg | $3,750/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody (Center). MBS9203985 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
M-CSF, Polyclonal Antibody (Cat# AAA9203985)
Full Name
M-CSF Antibody (Center)
Gene Names
CSF1; MCSF; CSF-1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-LAMP2 / CD107b antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
LAMP2 / CD107b, Monoclonal Antibody (Cat# AAA249477)
Full Name
Anti-LAMP2 / CD107b Antibody IHC-plus
Gene Names
LAMP2; LAMPB; CD107b; LAMP-2; LGP110
Reactivity
Mouse, Human
Applications
IHC, IF, WB
Purity
Protein G purified
Pricing
$610/0.2 mL | $2,685/5x0.2 mL
Goat anti Human IgG, Heavy and Light Chain Specific Antibody, Alkaline Phosphatase Conjugated, Secondary Antibody (Cat# AAA198062)
Full Name
Goat anti Human IgG, Heavy and Light Chain Specific Antibody, Alkaline Phosphatase Conjugated
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity chromatography
Pricing
$245/1 mg
Immunohistochemistry (IHC)
(Anti-ITGA6/Integrin Alpha 6/CD49f antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
ITGA6/Integrin Alpha 6/CD49f, Monoclonal Antibody (Cat# AAA249173)
Full Name
Mouse Monoclonal (IgG1) to Human ITGA6/Integrin Alpha 6/CD49f
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IHC - Paraffin, IF, WB, FC/FACS
Purity
Protein G Purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
(p53 Antibody (S315) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the p53 antibody detected the p53 protein (arrow).)
p53, Polyclonal Antibody (Cat# AAA9201927)
Full Name
p53 Antibody (S315)
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). MBS9208263 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
FGFR2, Polyclonal Antibody (Cat# AAA9208263)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data (TD)
( All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
BDNF, Polyclonal Antibody (Cat# AAA9211799)
Full Name
BDNF Antibody (C-term)
Gene Names
BDNF; ANON2; BULN2
Reactivity
Human
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Predicted Reactivity: Bovine, Horse, Mouse, Pig, Rat
Applications
WB, IHC-P, IF, FC, E
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(VIME Antibody western blot analysis in Hela,U251,A549,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the VIME antibody detected the VIME protein (arrow).)
Vimentin, Polyclonal Antibody (Cat# AAA9213000)
Full Name
Vimentin Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human
Applications
IF, EIA, WB, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
( All lanes : Anti-ROR1 Antibody at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: -MB-231 whole cell lysateLane 3: NCI-H226 whole cell lysateLane 4: A549 whole cell lysateLane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ROR1, Polyclonal Antibody (Cat# AAA9200685)
Full Name
ROR1 Antibody
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
FOLR1, Monoclonal Antibody (Cat# AAA605669)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
$850/0.1 mg | $3,815/5x0.1 mg
Western Blot (WB)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, MBS9610328, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated (MBS9612520) and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated (MBS9612517) secondary antibodies)
beta-Tubulin, Monoclonal Antibody (Cat# AAA9610328)
Full Name
beta-Tubulin Mouse Monoclonal Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Applications
WB, IHC, IF, ICC
Purity
Peptide affinity purification
Pricing
$190/0.05 mL | $245/0.1 mL | $310/0.2 mL | $855/1 mL | $3,800/5x1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
CB2, Polyclonal Antibody (Cat# AAA9203911)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
EIA, IHC, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(TERT Antibody (pS1125) western blot analysis in Jurkat cell line and human normal uterus tissue lysates (35ug/lane).This demonstrates the TERT antibody detected the TERT protein (arrow).)
TERT, Polyclonal Antibody (Cat# AAA9207567)
Full Name
TERT Antibody (S1125)
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
LGR5 (GPR49), Polyclonal Antibody (Cat# AAA9205024)
Full Name
LGR5 (GPR49) Antibody (Center)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
FC/FACS, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
