Rabbit anti-Human, Mouse ACTRIIB / ACVR2B Polyclonal Antibody | anti-ACTRIIB antibody

Rabbit Polyclonal to Human ACTRIIB / ACVR2B

Cat. Num. AAA247275

• Gene Names: ACVR2B; HTX4; ACTRIIB; ActR-IIB
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: ACTRIIB / ACVR2B; Polyclonal Antibody; Rabbit Polyclonal to Human ACTRIIB / ACVR2B; Anti-ACTRIIB / ACVR2B Antibody (N-Terminus) IHC-plus; ACVR2B; Activin A receptor type IIB; Activin receptor type-2B; ActR-IIB; ACTRIIB; Activin receptor type IIB; Activin A receptor; type IIB; HTX4; Human ACTRIIB; anti-ACTRIIB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This ACVR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected between aa 1 and 30 of human ACVR2B.
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 512

• Applicable Applications for anti-ACTRIIB antibody: Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P), Western Blot (WB)
• Application Notes: IHC; IHC-Paraffin (10 ug/ml); Western Blot (1:1000); Optimal dilution to be determined by the researcher.
• Immunogen Species: ACTRIIB / ACVR2B antibody was raised against Human.
• Epitope: aa1-30
• Target: ACTRIIB/ACVR2B
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-ACTRIIB / ACVR2B antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot of anti-ACVR2B Antibody (N-term) in mouse heart, kidney and brain lysates (35 ug/lane). ACVR2B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot of ACVR2B N-term S14 in Jurkat cell line lysates (35 ug/lane). ACVR2B (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ACTRIIB antibody

Family: Protein Kinase; Subfamily: Activin/TGFb

NCBI and Uniprot Product Information

• NCBI GI #: 116734708
• NCBI GeneID: 93
• NCBI Accession #: NP_001097.2
• NCBI GenBank Nucleotide #: NM_001106.3
• UniProt Accession #: Q13705; Q4VAV0
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin A receptor, type IIB
• NCBI Official Symbol: ACVR2B
• NCBI Official Synonym Symbols: HTX4; ACTRIIB; ActR-IIB
• NCBI Protein Information: activin receptor type-2B
• UniProt Protein Name: Activin receptor type-2B
• UniProt Gene Name: ACVR2B
• UniProt Synonym Gene Names: ACTR-IIB
• UniProt Entry Name: AVR2B_HUMAN

NCBI Description

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Membrane protein, integral; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Kinase, protein; EC 2.7.11.30; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; metal ion binding; growth factor binding; protein serine/threonine/tyrosine kinase activity; activin binding; activin receptor activity, type II; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; activin receptor signaling pathway; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; pancreas development; embryonic foregut morphogenesis; lymphangiogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; response to glucose stimulus; mesoderm development; kidney development; gastrulation with mouth forming second; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; organ growth; skeletal morphogenesis; insulin secretion; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; lung development

Disease: Heterotaxy, Visceral, 4, Autosomal