Rabbit Phospho-CDC25A (T507) Polyclonal Antibody | anti-CDC25A antibody

Phospho-CDC25A (T507) Antibody

Cat. Num. AAA9204880

• Gene Names: CDC25A; CDC25A2
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-CDC25A (T507); Polyclonal Antibody; Phospho-CDC25A (T507) Antibody; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; CDC25A; anti-CDC25A antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 484

• Applicable Applications for anti-CDC25A antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IF~~1:25; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF)

(Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. MBS9204880 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, untreated or treated with calyculin A, using Phospho-Cdc25A Antibody (T507).)

Western Blot (WB)

(The anti-Phospho-CDC25A-T507 Pab is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CDC25A antibody

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CDC25A antibody

Crown Antibodies; Cancer; Developmental Biology; Signal Transduction; Phospho Antibodies

References

Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). Nemoto, K., et al., Prostate 58(1):95-102 (2004). Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003). Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003). Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 42490758
• NCBI GeneID: 993
• NCBI Accession #: NP_001780.2; NP_963861.1
• NCBI GenBank Nucleotide #: NM_001789.2
• UniProt Accession #: P30304; Q8IZH5; Q96IL3; Q9H2F2
• Molecular Weight: 59087
• NCBI Official Full Name: M-phase inducer phosphatase 1 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25A
• NCBI Official Symbol: CDC25A
• NCBI Official Synonym Symbols: CDC25A2
• NCBI Protein Information: M-phase inducer phosphatase 1
• UniProt Protein Name: M-phase inducer phosphatase 1
• Protein Family: M-phase inducer phosphatase
• UniProt Gene Name: CDC25A
• UniProt Entry Name: MPIP1_HUMAN

NCBI Description

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: mitosis; cell proliferation; response to radiation; regulation of cell cycle; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle