Mouse anti-Human ITGA6/Integrin Alpha 6/CD49f Monoclonal Antibody | anti-ITGA6 antibody

Mouse Monoclonal (IgG1) to Human ITGA6/Integrin Alpha 6/CD49f

Cat. Num. AAA249173

• Gene Names: ITGA6; CD49f; VLA-6; ITGA6B
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: ITGA6/Integrin Alpha 6/CD49f; Monoclonal Antibody; Mouse Monoclonal (IgG1) to Human ITGA6/Integrin Alpha 6/CD49f; Anti-ITGA6/Integrin Alpha 6/CD49f Antibody IHC-plus; ITGA6; CD49f; CD49f antigen; Integrin alpha6B; Integrin; alpha 6; Integrin alpha-6 precursor; VLA-6; Integrin alpha-6; ITGA6B; Human ITGA6/Integrin Alpha 6/CD49f; anti-ITGA6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Human CD49f (IHC-P data suggests that this antibody may be specific for CD49f isoform X1X2).
• Purity/Purification: Protein G Purified
• Form/Format: Tissue Culture Supernatant, PBS, pH 7.2, 0.09% Sodium Azide
• Sequence Length: 1073

• Applicable Applications for anti-ITGA6 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: FC (1:10 - 1:50); IF (1:10 - 1:50); IHC-P (1:50); WB (1:100 - 1:16000)
• Target: Human ITGA6/Integrin Alpha 6/CD49f
• Immunogen Species: ITGA6/Integrin Alpha 6/CD49f antibody was raised against Human
• Epitope: aa324-620
• Immunogen: ITGA6/Integrin Alpha 6/CD49f antibody was raised against recombinant protein encoding amino acids 324-620
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C.

Immunohistochemistry (IHC)

(Anti-ITGA6/Integrin Alpha 6/CD49f antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Immunofluorescence (IF)

(Confocal immunofluorescence of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(ITA6 Monoclonal Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of anti-ITA6 in HepG2 cell line lysates (35 ug/lane). ITA6 (arrow) was detected using the purified Mab.(8 ug/ml))

Related Product Information for anti-ITGA6 antibody

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Product Categories/Family for anti-ITGA6 antibody

Family: Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 119395742
• NCBI GeneID: 3655
• NCBI Accession #: NP_000201.2
• NCBI GenBank Nucleotide #: NM_000210.2
• UniProt Accession #: P23229; Q08443; Q0MRC7; Q14646; Q16508; Q53RX7; Q59HB7; B2RMU9; B4DG69; B4DKB8; C4AM96; G5E9H1
• NCBI Official Full Name: integrin alpha-6 isoform b
• NCBI Official Synonym Full Names: integrin, alpha 6
• NCBI Official Symbol: ITGA6
• NCBI Official Synonym Symbols: CD49f; VLA-6; ITGA6B
• NCBI Protein Information: integrin alpha-6; integrin alpha6B; CD49 antigen-like family member F
• UniProt Protein Name: Integrin alpha-6
• Protein Family: Integrin
• UniProt Gene Name: ITGA6
• UniProt Synonym Gene Names: Alpha6p
• UniProt Entry Name: ITA6_HUMAN

NCBI Description

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cell-cell adherens junction; cell surface; focal adhesion; hemidesmosome; plasma membrane; basal plasma membrane; basement membrane; filopodium; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding

Biological Process: skin development; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cell-substrate junction assembly; brown fat cell differentiation; positive regulation of cell-cell adhesion; cellular response to extracellular stimulus; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia