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91 results for " Abs to IgG Lambda" - showing 1-50
Western Blot (WB)
(Western Blot analysis of PRKCI (arrow) using MBS624797 . 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies).)
PKC iota, Polyclonal Antibody (Cat# AAA624797)
Full Name
PKC iota, NT (Protein Kinase C iota Type, Atypical Protein Kinase C-lambda/iota, PRKC-lambda/iota, aPKC-lambda/iota, nPKC-iota, PRKCI, DXS1179E)
Gene Names
PRKCI; PKCI; DXS1179E; MGC26534; nPKC-iota
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified by ammonium sulfate precipitation
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from COS cells, treated with λ phosphatase or 20% FBS as indicated, using Non-phospho-4E-BP1 (Thr46) (upper), Phospho-4E-BP1 (Thr37/46) Antibody (middle), and 4E-BP1 Antibody (lower).)
4E Binding Protein 1, non-phosphorylated, Monoclonal Antibody (Cat# AAA633422)
Full Name
4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
Reactivity
Human, Monkey, Mouse, Rat
Applications
EL/EIA, WB, FC/FACS
Purity
Supernatant
Supernatant
Supernatant
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using MBS622361.)
TCTP, Polyclonal Antibody (Cat# AAA622361)
Full Name
TCTP, phosphorylated (Ser46) (Translationally controlled tumor protein, TCTP p23 HRF)
Gene Names
TPT1; TCTP; MGC128232
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, FC/FACS, IF
Purity
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Baculovirus-expressed PKC isoforms alpha, beta, gamma, delta and epsilon, untreated or treated with lambda protein phosphatase, using MBS630676 Phospho-PKCalpha/beta II (Thr638/641) Antibody (upper) or PKCalpha, beta, gamma, delta, epsilon antibodies (lower).)
Protein Kinase C, alpha, beta 2, Polyclonal Antibody (Cat# AAA630676)
Full Name
Protein Kinase C, alpha, beta 2, phosphorylated (Thr638/641) (PKCa,bII)
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Monkey, Mouse, Rat
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western Blot Analysis: Lysates from Lambda phosphatase-treated (lane 1) and untreated (lane 2) A375 cells were resolved by electrophoresis, transferred to PVDF and probed with MBS616305 (1:500). Proteins were visualized using a IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates Acetyl CoA Carboxylase (Ser79) (~257kD).)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA616305)
Full Name
AcCoA Carboxylase (Ser79), phosphorylated (ACC, Acetyl Coenzyme A Carboxylase)
Gene Names
Acly; AW538652; A730098H14Rik
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$720/0.2 mg | $3,230/5x0.2 mg
Western Blot (WB)
(Fig. 1a: Western blot analysis of extracts from A549(CCL-185) cells, untreated or stimulated with dexamethasone (100 nM for 1 hr), using MBS630617.)
Glucocorticoid Receptor, Polyclonal Antibody (Cat# AAA630617)
Full Name
Glucocorticoid Receptor, phosphorylated (Ser211)
Gene Names
NR3C1; GR; GCR; GRL; GCCR
Reactivity
Human
Applications
EL/EIA, WB, IP, ICC, IF
Purity
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
SDS-Page
(Up-regulation, Antibody-Peptide Competition and Phosphatase Treatment: Extracts of COS cells transfected with muscle MLCK unstimulated (1) or phosphorylated in vitro by 0.05 μg of PKA (per μg of extract) for 10 minutes at 37°C (2-6), were resolved by SDS-PAGE on a 4-12% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (1-5) or treated with lambda phophatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with MBS618405 for two hours at RT in 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal method.)
Myosin Light Chain Kinase, Smooth Muscle, Polyclonal Antibody (Cat# AAA618405)
Full Name
Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083; FLJ12216; DKFZp686I10125
Reactivity
Bovine, Mouse, Rabbit
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
SDS-Page
(Peptide Competition Extracts prepared from PC12 cells treated with nocozadole were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with FAK [pS732] antibody for two hours at room temperature in a 1% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-serine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to FAK [pS732] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The phospho signal also was absent after lambda ( ) phosphatase stripping (data not shown), verifying that the antibody is phospho specific.)
FAK, Polyclonal Antibody (Cat# AAA630226)
Full Name
FAK, phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Reactivity
Chicken, Frog, Human, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Kappa, Lambda Light Chain, Monoclonal Antibody (Cat# AAA609443)
Full Name
Kappa, Lambda Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (HRP)
Reactivity
Rat
Applications
EL/EIA
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$1,150/0.5 mg | $5,170/5x0.5 mg
Western Blot (WB)
(Western blot analysis of extracts from COLO 201 cells with or without 24 hours’ starvation in serum-free medium (lanes 1 and 2), immunoprecipitates from COLO 201 cells using v-Src antibody treated with or without lambda phosphatase (lanes 3 and 4) and NIH/3T3 cells treated with PDGF (50 ng/ml) for 0, 2 and 5 minutes (lanes 5, 6 and 7, respectively), using MBS625627 (upper) and v-Src antibody (lower).)
Src Family, Polyclonal Antibody (Cat# AAA625627)
Full Name
Src Family, phosphorylated (Tyr416)
Gene Names
Src64B; c-src; C-src1; CG7524; D-src; D-Src64B; Dm SRC1; DmelCG7524; DmHD-358; dsrc; dSrc; Dsrc; DSrc; Dsrc64; DSrc64; DSRC64; Dsrc64B; DSrc64B; HD-358; MRE5; src; Src; SRC 64B; src1; Src1; src64; Src64; src64b; src64B
Reactivity
Species Crossreactivity: Mouse, Rat
Species sequence homology: Xenopus 100%
Species sequence homology: Xenopus 100%
Applications
WB
Purity
Affinity Purified
Purified by Protein A chromatography and immunoaffinity chromatography.
Purified by Protein A chromatography and immunoaffinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using MBS632437 S6 Phosphorylated Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb MBS632437 (lower).)
S6 Ribosomal Protein, Monoclonal Antibody (Cat# AAA632437)
Full Name
S6 Ribosomal Protein, phosphorylated (Ser235/236)
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, FC/FACS, IF
Purity
As reported
Pricing
$435/0.02 mL | $745/0.1 mL | $3,340/5x0.1 mL
Western Blot (WB)
(Western Blot of NIH/3T3 cells. Lambda- phosphatase treated or untreated and various other cell lines using MBS601180)
Cofilin, Monoclonal Antibody (Cat# AAA601180)
Full Name
Cofilin, phosphorylated (Ser3)
Gene Names
CFL1; CFL
Reactivity
Bovine, Human, Monkey, Mouse, Rat
Applications
EL/EIA, WB, IF
Purity
Supernatant
Supernatant
Supernatant
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using MBS601384)
Akt Substrate, Monoclonal Antibody (Cat# AAA601384)
Full Name
Akt Substrate, phosphorylated (RXRXXS/T)
Gene Names
Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
Applications
EL/EIA, WB, IP, IHC
Purity
Supernatant
Ascites
Ascites
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Testing Data
(Peptide Competition and Phosphatase Treatment: Rat brain lysates were either left untreated (1-4) or treated with lambda (l) phosphatase (5). Lysates were then incubated with MBS625617, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). The data show that only the peptide corresponding to CaMKIIalpha [pT286] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Calmodulin-Dependent Protein Kinase II, alpha, Polyclonal Antibody (Cat# AAA625617)
Full Name
Calmodulin-Dependent Protein Kinase II, alpha, phosphorylated (Thr286) (CAMKII)
Gene Names
CAMK2A; CAMKA; KIAA0968
Reactivity
Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
IgG, Lambda, Polyclonal Antibody (Cat# AAA630224)
Full Name
IgG, Lambda
Reactivity
Human
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Pricing
$555/1 mg | $2,490/5x1 mg
Western Blot (WB)
(Western blot analysis of active p42 MAP Kinase, active p38 MAPK and active JNK2 proteins, as well as extracts from PDGF-treated NIH/3T3 cells and EGF-treated A431 cells, using MBS602140)
MAP Kinase p44/42, Monoclonal Antibody (Cat# AAA602140)
Full Name
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Reactivity
Drosophila, Human, Monkey, Mouse, Porcine, Rat0
Applications
WB, IP, IHC
Purity
Supernatant
Supernatant
Supernatant
Pricing
$650/0.2 mL | $2,905/5x0.2 mL
Western Blot (WB)
(IGLL5 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the IGLL5 Antibody detected the IGLL5 protein (arrow).)
IGLL5, Polyclonal Antibody (Cat# AAA6005397)
Full Name
IGLL5, CT (IGLL5, Immunoglobulin lambda-like polypeptide 5, G lambda-1, Germline immunoglobulin lambda 1)
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at RT, and incubated with MBS627363 for two hours at RT in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that the peptide corresponding to Rac1/cdc42 [pS71] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Rac1, Polyclonal Antibody (Cat# AAA627363)
Full Name
Rac1, phosphorylated (Ser71)
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Western Blot (WB)
(The anti-IL28 Ctr-term Pab is used in Western blot to detect IL28 in mouse brain tissue lysate.)
IL28, Polyclonal Antibody (Cat# AAA625928)
Full Name
IL28, ID (Interleukin-28A, IL-28A, Interferon lambda-2, IFN-lambda-2, Cytokine ZCYTO20, IFNL2, ZCYTO20, IL28A)
Gene Names
Ifnl3; Il28; IFL-1; Il28b; IL-28B; INF-alpha; INF-lambda
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Testing Data
(Peptide Competition and Phosphatase Stripping: Extracts prepared from NIH3T3 cells stably expressing c-Src Y527F mutant protein were left untreated (1-4) or treated with lambda (l) phosphatase (5). Extracts were incubated with 0.35 ug/mL MBS615537)
Cortactin, Polyclonal Antibody (Cat# AAA615537)
Full Name
Cortactin, phosphorylated (Tyr466)
Gene Names
CTTN; EMS1; FLJ34459
Reactivity
Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
IgG, Lambda, Polyclonal Antibody (Cat# AAA628868)
Full Name
IgG, Lambda (Biotin)
Reactivity
Mouse
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$470/0.5 mg | $2,105/5x0.5 mg
Western Blot (WB)
(Western Blot analysis of A431 cells, untreated or calyculin A-treated, using MBS627817.)
Protein Kinase A, Substrate, Polyclonal Antibody (Cat# AAA627817)
Full Name
Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)
Applications
EL/EIA, WB, IP, IHC
Purity
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Purified by protein A and peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from various cell types, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using MBS611166 CDK9 Phosphotylated (Thr186) Antibody (upper) or CDK9 (C12F7) Rabbit mAb MBS611166 (lower).)
cdk9, p43, Polyclonal Antibody (Cat# AAA611166)
Full Name
cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9), phosphorylated (Thr186)
Gene Names
Cdk9; cdk9; CDK9; CG5179; DmelCG5179; P-TEF; P-TEFb; PTefb
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB
Purity
Purified using peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases using Phospho-Talin (Ser425) Antibody #MBS6008054 (upper) or Talin-1 Rabbit mAb (lower).)
Talin, Polyclonal Antibody (Cat# AAA6008054)
Full Name
Talin, phosphorylated (Ser425) (TLN, Talin 1, Talin-1, TLN1, ILWEQ, KIAA1027)
Gene Names
TLN1; TLN; ILWEQ
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
IgG, Lambda, Light Chain, Monoclonal Antibody (Cat# AAA608956)
Full Name
IgG, Lambda, Light Chain
Reactivity
Human
Applications
EL/EIA
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$325/0.1 mg | $1,460/5x0.1 mg
Protein Kinase C, iota, lambda, Polyclonal Antibody (Cat# AAA627198)
Full Name
Protein Kinase C, iota, lambda, phosphorylated (Thr555/Thr563) (PKCi, PKC, Atypical Protein Kinase C lambda/iota, aPKC lambda iota, PRKCL, DXS1179E, MGC26534)
Gene Names
PRKCI; PKCI; DXS1179E; nPKC-iota
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$715/10 Blots | $3,205/5x10 Blots
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from NIH3T3 cells stimulated with PDGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda (λ) phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with MBS625189for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that the peptide corresponding to CREB [pSpS129/133] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific. Pretreatment of cells with PKA inhibitor (PKI) or GSK-3 β inhibitor (LiCl) diminished the signal, indicating the dual phosphoreactivity of this antibody (data not shown).)
CREB, Polyclonal Antibody (Cat# AAA625189)
Full Name
CREB, phosphorylated (Ser129, Ser133) (cAMP Response Element Binding Protein)
Gene Names
CREB1; CREB; MGC9284
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated CREB.
Purified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated CREB.
Pricing
$875/0.1 mL | $3,920/5x0.1 mL
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from NIH3T3 cells stimulated with PDGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at RT, and incubated with MBS630388 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide)
CREB, Polyclonal Antibody (Cat# AAA630388)
Full Name
CREB, phosphorylated (Ser129, Ser133) (cAMP Response Element Binding Protein)
Gene Names
CREB1; CREB; MGC9284
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Affinity chromatography.
Purified by Affinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Western Blot (WB)
(Western blot analysis of lambda protein phosphatase (lambda-PPase) treated cdc2 kinase, using MBS623445)
cdc2, Polyclonal Antibody (Cat# AAA623445)
Full Name
cdc2, phosphorylated (Thr161)
Gene Names
cdc2; 5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IP, ICC, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) Rabbit mAb #MBS644659 (upper) and α/β-Tubulin Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).)
Glycogen Synthase Kinase 3 beta, Monoclonal Antibody (Cat# AAA644659)
Full Name
Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (Glycogen Synthase Kinase-3 beta, GSK-3 beta, GSK3B)
Reactivity
Hamster, Human, Mouse, Rat
Applications
WB, IP, FC/FACS, IF
Purity
Purified
Purified
Purified
Pricing
$435/0.02 mL | $745/0.1 mL | $3,340/5x0.1 mL
Immunoblot (IB)
(Immunoblot Analysis Lysates from A431 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- MEK1 (Thr292) for 1 hours at RT Lane 2 was preincubated with lambda phosphatase prior to primary antibody incubation. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-MEK1.)
MEK1, Polyclonal Antibody (Cat# AAA625911)
Full Name
MEK1, phosphorylated (Thr292) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)
Gene Names
MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
Reactivity
Hamster, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$700/0.1 mL | $3,135/5x0.1 mL
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates from HepG2 cells stimulated with 8 ng/mL TGF£] for 60 minutes were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for two hours at RT and either left untreated (1-4) or treated with lambda (£f) phosphatase (5), then incubated with MBS613106 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to Smad2 [pSpS465/467] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Smad2, Polyclonal Antibody (Cat# AAA613106)
Full Name
Smad2, phosphorylated (Ser465, Ser467) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, lambda phosphatase treated (lane 2) or untreated (lane 3), using MBS624387.)
Glycogen Synthase Kinase 3 beta, Polyclonal Antibody (Cat# AAA624387)
Full Name
Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)
Reactivity
Human, Monkey, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(IL28B Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the IL28B antibody detected the IL28B protein (arrow).)
IL28B, Polyclonal Antibody (Cat# AAA643503)
Full Name
IL28B, NT (IL28B, IFNL3, IFNL4, IL28C, ZCYTO22, Interleukin-28B, Cytokine Zcyto22, Interferon lambda-3, Interferon lambda-4, Interleukin-28C)
Gene Names
IFNL3; IL28B; IL28C; IL-28B
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB)
(WB: Lysates from estradiol and EGF treated MCF-7 cells (lanes 1 and 2) or recombinant estrogen receptor alpha protein phosphorylated with MAPK2/Erk2 (lanes 3 and 4) were either treated with lambda phosphatase (lanes 1 and 3) or left untreated (lanes 2 and 4), then probed with MBS609358 (1:2000 dilution).)
Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA609358)
Full Name
Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$695/0.1 mL | $3,110/5x0.1 mL
Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping: Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were either untreated (1-6), or treated with lambda phosphatase (7). Extracts were t)
Raf, Polyclonal Antibody (Cat# AAA628216)
Full Name
Raf, phosphorylated (Ser259) (Raf-1, c-Raf)
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$715/10 Blots | $3,205/5x10 Blots
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates of K562 cells stimulated with PMA were either left untreated (1-4) or treated with lambda (l) phosphatase (5) then incubated with PKCbetaI&II [pT500] antibody (2h, RT) following prior incubation with:)
Protein Kinase C, beta1, beta2, Polyclonal Antibody (Cat# AAA615155)
Full Name
Protein Kinase C, beta1, beta2, phosphorylated (Thr500) (PKCb1, PKCb2)
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Chemiluminescence
(Representative lot data. Untreated (lane 1) and lambda phosphatase- treated (lane 2), hypotonic-stimulated HEK293 cell lysates were probed with MBS6004201 (0.5ug/ml). Proteins were visualized using a IgG (HRP)donkey anti-rabbit Iand a chemiluminescence detection system. Arrow indicates SPAK (Ser373) / OSR1 (Ser325) (~60kD).)
SPAK, Polyclonal Antibody (Cat# AAA6004201)
Full Name
SPAK, Phosphorylated (Ser373), OSR1, Phosphorylated (Ser325) (STE20/SPS1-related Proline-alanine-rich Protein Kinase, Ste-20-related Kinase, DCHT, Serine/Threonine-protein Kinase 39, STK39/ Serine/Threonine-protein Kinase OSR1, Oxidative Stress-responsive
Reactivity
Species Sequence Homology: Rat and canine, 100%
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$660/0.1 mg | $2,965/5x0.1 mg
Immunohistochemistry (IHC)
(Human Spleen (formalin-fixed, paraffin-embedded) stained with CAMK2 antibody at 1:200 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Calmodulin-Dependent Protein Kinase II, Polyclonal Antibody (Cat# AAA648884)
Full Name
Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2)
Gene Names
CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
Reactivity
Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$735/0.05 mL | $3,290/5x0.05 mL
Western Blot (WB)
(Western Blot/Peptide Inhibition Analysis: Representative lot data. HeLa cell lysates were probed with MBS616256 (1:1000) (Lane 1), or with the addition of non-phospho peptide (Lane 2), or phospho peptide (Lane 3). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho- BMK1/Erk5 (Thr218/Tyr220) (~115kD).)
ERK5, Polyclonal Antibody (Cat# AAA616256)
Full Name
ERK5, phosphorylated (Thr218, Tyr220) (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$760/0.2 mL | $3,415/5x0.2 mL
Western Blot (WB)
(IGL@ polyclonal antibody. Western Blot analysis of IGL@ expression in human liver.)
IGL, Polyclonal Antibody (Cat# AAA6000672)
Full Name
IGL@ (IGL, MGC88804, Immunoglobulin lambda Locus, IGL, IGLC6, MGC88804)
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$620/0.05 mg | $2,775/5x0.05 mg
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-alpha (3-7) were either left untreated (1-6) or treated with lambda (l) phosphatase (7). Lysates were then incubated with MAPKAPK-2 [pT334] antibody, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific)
MAPKAP Kinase 2, Polyclonal Antibody (Cat# AAA626880)
Full Name
MAPKAP Kinase 2, phosphorylated (Thr334) (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
Gene Names
MAPKAPK2; MK2
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$695/10 Blots | $3,110/5x10 Blots
Western Blot (WB)
(Western Blot Analysis: Rat brain microsomal tissue lysate was probed with MBS629605 (1ug/ml). Proteins were visualized using an IgG (HRP) Donkey Anti-Rabbit and chemiluminescence detection system. Arrow indicates NR1 (~120kD).)
NR1, Polyclonal Antibody (Cat# AAA629605)
Full Name
NR1, phosphorylated (Ser897) (N-methyl-D-aspartate Receptor subunit NR1, GRIN1, NMDA1, NMDAR1, NMD-R1, Glutamate receptor ionotropic N-methyl D-aspartate 1)
Reactivity
Rat
Applications
WB, IP, IHC
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$875/0.1 mg | $3,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using MBS613740 HP1γ Phosphorylated (Ser83) Antibody (upper panel) or HP1γ Antibody MBS613740 (lower panel).)
Heterochromatin Protein 1, gamma, Polyclonal Antibody (Cat# AAA613740)
Full Name
Heterochromatin Protein 1, gamma (HP1g, M32), phosphorylated (Ser83)
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IP, IF
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(WB: untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with MBS629384 (1:1000).)
NOS III, Polyclonal Antibody (Cat# AAA629384)
Full Name
NOS III, phosphorylated (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)
Gene Names
NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Reactivity
Bovine, Human
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$930/0.1 mL | $4,175/5x0.1 mL
IgG, lambda chain, Polyclonal Antibody (Cat# AAA624400)
Full Name
IgG, lambda chain (HRP)
Reactivity
Human
Applications
EL/EIA, WB, IHC, GS/EMSA
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$660/1 mg | $2,965/5x1 mg
Western Blot (WB)
(Western blot to detect IL29 in Jurkat cell lysate using MBS630878.)
IL29, Polyclonal Antibody (Cat# AAA630878)
Full Name
IL29, ID (Interleukin-29, IL-29, Interferon lambda-1, IFN-lambda-1, Cytokine ZCYTO21, FNL1, ZCYTO21, IL29)
Gene Names
IL29; IFNL1; IL-29
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Western Blot (WB)
(Western Blot Analysis Untreated (Lane 1) or PDGF treated (Lanes 2, 3, 4) lysates from NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-PRAS40 (Thr246) for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho- PRAS40 (Thr246). Lane 4 blot was pre-incubated with λ phosphatase. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-PRAS40 (Thr246).)
PRAS40, Polyclonal Antibody (Cat# AAA613629)
Full Name
PRAS40, phosphorylated (Thr246) (Proline-Rich AKT Substrate 40kD Lobe, AKT151)
Gene Names
AKT1S1; Lobe; PRAS40
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$705/0.1 mL | $3,170/5x0.1 mL
Western Blot (WB)
(Western blot analysis of recombinant human PKC isoforms using Rabbit Anti-PKCι/λ Polyclonal Antibody , demonstrating the isoform-specificity of this antibody.)
Protein Kinase C, iota, lambda, Polyclonal Antibody (Cat# AAA626916)
Full Name
Protein Kinase C, iota, lambda (PKCi, PKC, Atypical Protein Kinase C lambda/iota, aPKC lambda iota, PRKCL, DXS1179E, MGC26534)
Gene Names
PRKCI; PKCI; DXS1179E; nPKC-iota
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$420/0.04 mg | $1,885/5x0.04 mg
Western Blot (WB)
(Western blot analysis of lysates from HeLa, NIH/3T3 and C6 cells treated for 24 hours with or without nocodazole (0.1 mg/ml) and also with or without λ phosphatase, using MBS633113 (upper) or Histone H3 Antibody MBS633113 (lower).)
Histone H3, Monoclonal Antibody (Cat# AAA633113)
Full Name
Histone H3, phosphorylated (Thr11)
Reactivity
Human
Applications
WB, IP, FC/FACS
Purity
Purified
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
