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Western Blot (WB)
(Western blot analysis of lambda protein phosphatase (lambda-PPase) treated cdc2 kinase, using MBS623445)
Rabbit cdc2 Polyclonal Antibody | anti-cdc2 antibody
cdc2, phosphorylated (Thr161)
Gene Names
cdc2; 5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
cdc2; Polyclonal Antibody; phosphorylated (Thr161); Anti -cdc2; anti-cdc2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of cdc2 only when phosphorylated at Thr161. Crossreacts with endogenous cdk2 phosphorylated at Thr160. Species Crossreactivity: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-cdc2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immufluorescence.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunocytochemistry (for IF): 1:200
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunocytochemistry (for IF): 1:200
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2 (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of lambda protein phosphatase (lambda-PPase) treated cdc2 kinase, using MBS623445)
Western Blot (WB)
(Western blot analysis of lambda protein phosphatase (lambda-PPase) treated cdc2 kinase, using MBS623445)
Western Blot (WB)
(Western blot analysis of HeLa samples immunoprecipitated with MBS623445and then probed with control cdc2 (POH1) Monoclonal Antibody #9116.)
Western Blot (WB)
(Western blot analysis of HeLa samples immunoprecipitated with MBS623445and then probed with control cdc2 (POH1) Monoclonal Antibody #9116.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells synchronized and enriched for different stages of the cell cycle, and probed with MBS623445(upper) or control cdc2 (POH1) Monoclonal Antibody #9116 (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells synchronized and enriched for different stages of the cell cycle, and probed with MBS623445(upper) or control cdc2 (POH1) Monoclonal Antibody #9116 (lower).)
Immunofluorescence (IF)
(Dual immunofluorescent staining of growing HeLa cells, using cdc2 (POH1) Monoclonal Antibody (left) or MBS623445(right).)
Immunofluorescence (IF)
(Dual immunofluorescent staining of growing HeLa cells, using cdc2 (POH1) Monoclonal Antibody (left) or MBS623445(right).)
Related Product Information for anti-cdc2 antibody
Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161 (1-4). The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of tyrosine 15 and threonine 14 (3,5). Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases. Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (3,4,6). cdc2 activation requires phosphorylation at Thr161, which is attributed to the cdk-activating kinase CAK, a complex of cdk7 and cyclin H (7). Phosphorylation at Thr161 is also required for association with cyclin A (8).
Product Categories/Family for anti-cdc2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cdc2
NCBI Official Symbol
cdc2
NCBI Official Synonym Symbols
5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
NCBI Protein Information
CG5363 gene product from transcript CG5363-RA; cdc2; CG5363-PA; cdc2-PA; cyclin-dependent kinase 1; CDC2 kinase; cyclin dependent kinase
Protein Family
Research Articles on cdc2
Similar Products
Product Notes
The cdc2 (Catalog #AAA623445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cdc2, phosphorylated (Thr161) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's cdc2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immufluorescence. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunocytochemistry (for IF): 1:200. Researchers should empirically determine the suitability of the cdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cdc2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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