Immunohistochemistry (IHC)
(Human Spleen (formalin-fixed, paraffin-embedded) stained with CAMK2 antibody at 1:200 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Rat Calmodulin-Dependent Protein Kinase II Polyclonal Antibody | anti-CaMKII antibody
Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2)
Gene Names
CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Calmodulin-Dependent Protein Kinase II; Polyclonal Antibody; Phosphorylated (Thr286) (CaM Kinase II; CaMKII; CaMK-II; CAMK2); Anti -Calmodulin-Dependent Protein Kinase II; anti-CaMKII antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
Recognizes rat CAMK2 when Phosphorylated at Thr286. Species sequence homology: Xenopus, human and mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5,150mM sodium chloride,100ug/ml BSA, 50% glycerol.
Applicable Applications for anti-CaMKII antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:200
Dilution: Western Blot: 1:1000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:200
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding the Thr286 found in rat brain CaM Kinase II.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Spleen (formalin-fixed, paraffin-embedded) stained with CAMK2 antibody at 1:200 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(Human Spleen (formalin-fixed, paraffin-embedded) stained with CAMK2 antibody at 1:200 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Western Blot (WB)
(Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.)
Western Blot (WB)
(Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.)
Related Product Information for anti-CaMKII antibody
CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1.
Product Categories/Family for anti-CaMKII antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Calcium/calmodulin-dependent protein kinase II, isoform M
NCBI Official Synonym Full Names
Calcium/calmodulin-dependent protein kinase II
NCBI Official Symbol
CaMKII
NCBI Official Synonym Symbols
Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
NCBI Protein Information
CG18069 gene product from transcript CG18069-RK; CG18069-PA; CG18069-PB; CG18069-PC; CG18069-PD; CG18069-PE; CG18069-PG; CG18069-PH; CG18069-PI; CG18069-PJ; CG18069-PK; CG18069-PL; CG18069-PM; CaM kinase; CaM kinase II; CaM-kinase-II; CaMKII-PA; CaMKII-PB; CaMKII-PC; CaMKII-PD; CaMKII-PE; CaMKII-PG; CaMKII-PH; CaMKII-PI; CaMKII-PJ; CaMKII-PK; CaMKII-PL; CaMKII-PM; Ca[2+]/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; Calcium/calmodulin-dependent protein kinase II; calcium/ calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II; calmodulin kinase II; calmodulin-dependent kinase; lethal (4) 102EFb
Protein Family
Research Articles on CaMKII
Similar Products
Product Notes
The CaMKII (Catalog #AAA648884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin-Dependent Protein Kinase II, Phosphorylated (Thr286) (CaM Kinase II, CaMKII, CaMK-II, CAMK2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin-Dependent Protein Kinase II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:1000 Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:200. Researchers should empirically determine the suitability of the CaMKII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin-Dependent Protein Kinase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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