Rabbit Heterochromatin Protein 1, gamma Polyclonal Antibody | anti-CBX3 antibody

Heterochromatin Protein 1, gamma (HP1g, M32), phosphorylated (Ser83)

Cat. Num. AAA613740

• Gene Names: CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Heterochromatin Protein 1; gamma; Polyclonal Antibody; gamma (HP1g; M32); phosphorylated (Ser83); Anti -Heterochromatin Protein 1; anti-CBX3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Binds to coding regions of active genes (14). This phosphorylation impairs the ability of HP1 to silence transcription and may be a marker for transcription elongation (14).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-CBX3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.; Dilution: Immunofluorescence: 1:200; Western Blot: 1:1000; Immunoprecipitation: 1:25
• Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to amino acids surrounding Ser83 of human HP1gamma
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using MBS613740 HP1γ Phosphorylated (Ser83) Antibody (upper panel) or HP1γ Antibody MBS613740 (lower panel).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells, untreated (left), forskolin- and IBMX-treated (center) or λ phosphatase-treated (right), using MBS613740 HP1γ Phosphorylated (Ser83) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-CBX3 antibody

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (, and) are primarily associated with centromeric heterochromatin; however, HP1 and also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25kD in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation (12-14). HP1 is phosphorylated on Ser83 by protein kinase A (PKA) in vitro, and activation of PKA by forskolin and IBMX treatment leads to increased phosphorylation in vivo (14). Phosphorylation of HP1 on Ser83 also increases in mitosis as demonstrated by our Phospho-HP1 (Ser83) Antibody, which shows increased immunofluorescent staining in untreated mitotic cells and increased Western blot signal in lysates from cells arrested in mitosis by treatment with paclitaxel. Phosphorylation of Ser83 only occurs on a subpopulation of HP1 found associated with euchromatin,

Product Categories/Family for anti-CBX3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5732187
• NCBI GeneID: 11335
• UniProt Accession #: Q13185; Q96CD7; Q99409; Q9BVS3; Q9P0Z6
• Molecular Weight: 20,811 Da
• NCBI Official Full Name: Heterochromatin protein 1 gamma
• NCBI Official Synonym Full Names: chromobox homolog 3
• NCBI Official Symbol: CBX3
• NCBI Official Synonym Symbols: HECH; HP1-GAMMA; HP1Hs-gamma
• NCBI Protein Information: chromobox protein homolog 3; HP1 gamma homolog; OTTHUMP00000122519; OTTHUMP00000122520; OTTHUMP00000202463; modifier 2 protein; heterochromatin-like protein 1; heterochromatin protein HP1 gamma; heterochromatin protein 1 homolog gamma; chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• UniProt Protein Name: Chromobox protein homolog 3
• Protein Family: Chromobox protein
• UniProt Gene Name: CBX3
• UniProt Entry Name: CBX3_HUMAN

NCBI Description

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Uniprot Description

CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleoplasm; nuclear inner membrane; nuclear envelope; spindle; nuclear heterochromatin; nucleus; chromatin

Molecular Function: identical protein binding; protein domain specific binding; protein binding; enzyme binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent