Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping: Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were either untreated (1-6), or treated with lambda phosphatase (7). Extracts were t)
Rabbit anti-Human Raf Polyclonal Antibody
Raf, phosphorylated (Ser259) (Raf-1, c-Raf)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 0.1-1ug/ml
Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping: Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were either untreated (1-6), or treated with lambda phosphatase (7). Extracts were t)
Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping: Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were either untreated (1-6), or treated with lambda phosphatase (7). Extracts were t)
Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either untreated (1-6), or treated with lambda (λ) phosphatase (7), blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with 0.50 µg/mL c-Raf [pS259] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6, 7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to c-Raf [pS259] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phosphospecific.)
Immunoprecipitation (IP)
(Peptide Competition and Phosphatase Stripping Total cell lysates (1) or immunoprecipitates (2-7) prepared from Hek293 cells overexpressing c-Raf and stimulated with EGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either untreated (1-6), or treated with lambda (λ) phosphatase (7), blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with 0.50 µg/mL c-Raf [pS259] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6, 7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to c-Raf [pS259] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phosphospecific.)
NCBI and Uniprot Product Information
Uniprot Description
Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.
Similar Products
Product Notes
The Raf (Catalog #AAA628216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raf, phosphorylated (Ser259) (Raf-1, c-Raf) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Raf can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1-1ug/ml. Researchers should empirically determine the suitability of the Raf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
