Western Blot (WB)
(Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases using Phospho-Talin (Ser425) Antibody #MBS6008054 (upper) or Talin-1 Rabbit mAb (lower).)
Rabbit Talin Polyclonal Antibody | anti-TLN1 antibody
Talin, phosphorylated (Ser425) (TLN, Talin 1, Talin-1, TLN1, ILWEQ, KIAA1027)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases using Phospho-Talin (Ser425) Antibody #MBS6008054 (upper) or Talin-1 Rabbit mAb (lower).)
Western Blot (WB)
(Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases using Phospho-Talin (Ser425) Antibody #MBS6008054 (upper) or Talin-1 Rabbit mAb (lower).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
Uniprot Description
Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol
Molecular Function: integrin binding; actin filament binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding
Biological Process: axon guidance; platelet activation; intercellular junction assembly; cytoskeletal anchoring; unfolded protein response; platelet degranulation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cell motility; blood coagulation
Research Articles on TLN1
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Product Notes
The TLN1 tln1 (Catalog #AAA6008054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Talin, phosphorylated (Ser425) (TLN, Talin 1, Talin-1, TLN1, ILWEQ, KIAA1027) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Talin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the TLN1 tln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Talin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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