Rabbit Protein Kinase A, Substrate Polyclonal Antibody | anti-ypkA antibody

Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)

Cat. Num. AAA627817

• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Protein Kinase A; Substrate; Polyclonal Antibody; Phosphorylated (Ser/Thr) (PKA); Anti -Protein Kinase A; anti-ypkA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position. It is a useful tool in identifying new substrates of AGC family kinases, including PKA and PKC. It does not recognize the non-phosphorylated PKA substrate motif.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-ypkA antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100; Immunohistochemistry (Paraffin): 1:100; ELISA: 1:500
• Immunogen: Synthetic phospho- PKA substrate peptide.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of A431 cells, untreated or calyculin A-treated, using MBS627817.)

Western Blot (WB)

(Western Blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using MBS627817.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using MBS627817.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS627817.)

Related Product Information for anti-ypkA antibody

A class of kinases that regulates an array of physiological processes and includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase, protein kinase C, Akt and RSK shares a substrate specificity characterized by arginine at position -3 relative to the phosphorylated Ser or Thr (1,2). These are referred to as Arg-directed kinases or AGC-family kinases. Phospho-(Ser/Thr) PKA Substrate Antibody provides a powerful new tool for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

Product Categories/Family for anti-ypkA antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 31795319
• NCBI GeneID: 1149139
• NCBI Accession #: NP_857776.1
• NCBI GenBank Nucleotide #: NC_004836.1
• NCBI Official Full Name: protein kinase A
• NCBI Official Symbol: ypkA
• NCBI Protein Information: similar to Yersinia pseudotuberculosis protein kinase A
• Protein Family: Protein kinase